粒细胞集落刺激因子动员对供者外周血细胞树突状细胞亚群的影响

Effect of G-CSF on dendritic cell subsets in donor's mobilized peripheral blood cells

目的:研究粒细胞集落刺激因子(G-CSF)动员对外周血单个核细胞(PBMNC)中树突状细胞(DC)亚群及其功能的影响。方法:以CD34-Lin-HLA-DR+细胞群设门4色荧光分析方法检测25例无关供者G-CSF动员前、后外周血细胞DC亚群;ELISA检测其血清相关细胞因子IL-12p40、IL-10、IFN-γ、IL-4活性,并分析DC1/DC2(CD11c+CD123-/CD11c-CD123+)比值与CD34+细胞含量的相关性。结果:G-CSF动员后,供者PBMNC的DC1/DC2比值显著低于动员前(P<0.05);DC1/DC2与CD34+细胞含量呈负相关(r=-0.438,P<0.05),CD34+细胞/MNC≥0.4%时,DC1/DC2倒置;而血清相关细胞因子IL-12p40、IL-10、IFN-γ、IL-4水平无显著改变(P>0.05);DC2HLA-DR表达上调而CD83不表达。结论:G-CSF动员后,供者外周血CD34+细胞数量增加的同时,DC2数量也增加,这些DC2尽管HLA-DR表达上调,但仍处前体细胞状态,不直接分泌或调节Th2细胞分泌免疫抑制因子。

AIM ： To explore the effect of granulocyte colony stimulating factor （ G - CSF） on donor＇s dendritic cell （DC）subsets in peripheral blood cells （PBC）. METHODS： The subsets of dendritic cells in PBC were analyzed by CD34 ^- Lin^- HLA - DR^＋ cells and the levels of IL - 12p40, IL - 10, IFN -γ, IL -4 in the serum were tested by ELISA before （25 cases） or after G- CSF mobiling. The relationship between the ratio of DC1/DC2 （CD11c^＋ CD123^-/CD11c^- CD123^＋ ） and CD34^＋/MNC was explored. RESULTS： CD34^＋/MNC in PBC harvests was above 0.4% in 23 cases, and the ratio of DC1/DC2 was lower, the HLA - DR expression of DCz was enhanced after G - CSF mobiling than before, but the levels of IL - 12p40, IL - 10, IFN - γ, IL - 4 in donors serum and CD83 expression of DC2 were not changed by G - CSF. CONCLUSION： DC2 increased accompanied by the increase in CD34^＋ cells in the PBC harvests. Although the expression of HLA - DR in DC2 was up - regulated by G - CSF, these DC2 did not regulate Th2 cells to excrete inhibitor cell factors and kept on the precursor characters.