胃癌组织中EGFR和COX-2表达的意义及其相关性

Expression of epidermal growth factor receptor and cyclooxygenase-2 and their correlations in gastric carcinoma

目的：探讨表皮生长因子受体（EGFR）和环氧化酶-2（COX-2）的表达与胃癌侵袭、转移因素的关系以及EGFR与COX-2在胃癌组织中表达的相关性．方法：用免疫组化的方法检测61例胃癌组织和相应的20例癌旁组织石蜡切片中EGFR、COX-2表达情况；用Western blot方法检测10例胃癌组织和相应的癌旁组织中EGFR、COX-2蛋白的表达．结果：免疫组化检测胃癌组织中EGFR及COX-2的阳性表达率分别为36．07％、59．02％均明显高于癌旁组织的0％、25％（x^2=9．903，P〈0．01：x^2=6．972，P〈0．01）；COX-2表达与浸润深度、淋巴结转移、TNM分期和病理分化程度有关（x^2=6．333，P〈O．05；x^2=5．588，P〈0．05；x^2=8．826，P〈0．01；x^2=5．653，P〈0．05）．EGFR表达与淋巴结转移和TNM分期有关（f=10．648，P〈0．01;x^2=4．150，P〈0．05）．EGFR与COX-2表达呈明显相关（r=0．316，P〈0．05）．Western blot方法检测胃癌组织中EGFR及COX-2的蛋白表达高于癌旁组织（35．89±12．50vs15．14±2．15，P〈0．01：51．29±23．42vs27．65±7．42．P〈0．05）．结论：EGFR、COX-2在胃癌组织中高表达，EGFR、COX-2的表达与胃癌的侵袭、转移密切有关，COX-2表达与EGFR显著相关．

AIM： To investigate the expression of epidermal growth factor receptor （EGFR） and cyclooxygenase-2 （COX-2） as well as their correlations in gastric carcinoma. METHODS： Immunohistochemistry Was performed to detect the expression of EGFR and COX-2 in 61 cases of gastric cancer and 20 cases of cancer-adjacent tissues, while Western blot was used to examine the protein expression of EGFR and COX-2 in 10 cases of gastric cancer and their corresponding cancer-adjacent tissues. RESULTS： Immunohistochemistry showed that the expression of EGFR and COX-2 in gastric carcinoma were significantly higher than those in normal mucosa （36.07% vs 0%, x^2 = 9.903, P 〈 0.01; 59.02% vs 25%, x^2 = 6.972, P 〈 0.01）. COX-2 expression was correlated with the depth of invasion, lymph node metastasis, TNM classification and pathologic differentiation degree （ x^2 =6.333, P 〈 0.05; x^2 = 5.588, P 〈 0.05; x^2 = 8.826, P 〈 0.01; x^2 = 5.653, P 〈 0.05）. EGFR expression was correlated with lymph node metastasis, and TNM classification （ x^2 = 10.648, P 〈 0.01; x^2 = 4.150, P 〈 0.05）. There was also significant correlation between COX-2 and EGFR expression （r = 0.316, P 〈 0.05）. Western blot also showed that the protein expression of EGFR and COX-2 in gastric carcinoma were significantly higher than those in normal mucosa （35.89 ± 12.50 vs 15.14 ± 2.15, P 〈 0.01; 51.29 ± 23.42 vs 27.65 ± 7.42, P 〈 0.05）. CONCLUSION： EGFR and COX-2 are overexpressed in gastric carcinoma, which is associated with the invasion and metastasis of gastric cancer. COX-2 expression is positively correlated with EGFR expression.