干、鲜壁虎冻干粉对小鼠H22肝癌体内外抑制作用

Anti-tumor effects of dry and fresh Gekko Swinhonis Gunther freeze-dried powders on mouse H22 hepatocellular carcinoma in vivo and in vitro

目的:研究干、鲜壁虎冻干粉对荷H22实体型移植瘤小鼠体内抑瘤作用及其含药血清体外对H22肝癌细胞的增殖抑制作用.方法:采用昆明小鼠H22移植瘤模型观察干、鲜壁虎冻干粉体内抑瘤活性及小鼠胸腺、脾脏指数变化:MTT法检测不同浓度干、鲜壁虎冻干粉含药血清对H22肝癌细胞体外杀伤作用.结果:干、鲜壁虎低、中、高3个剂量组的抑瘤率分别为25.6%,40.0%,48.5%和20.7%,28.1%,51.1%.各壁虎组与西药5-FU组相比,不同程度提高了荷瘤小鼠的胸腺质量(P<0.01)、胸腺指数(P<0.05或P<0.01)和脾脏质量(P<0.05或P<0.01)、脾脏指数(P<0.05).含药血清在体外能够明显抑制H22细胞的增殖,干、鲜壁虎低、中、高含药血清组的增殖抑制率为17.4%,21.0%,34.5%和16.4%,26.3%,43.2%.结论:干、鲜壁虎冻干粉均具有良好的抗肿瘤作用.

AiM： To investigate the anti-tumor activity of dry and fresh Gekko Swinhonis Gunther freezedried powder （DGFP and FGFP） on mouse H22 hepatocellular carcinoma in vivo and in vitro. METHODS： The mice bearing H22 tumor cells were used in this study. After treatment with DGFP and FGFP in vivo and in vitro, the antitumor effects as well as the weights of thymus and spleen were measured, and the proliferation of H22 cells was detected by MTT assay. RESULTS： The tumor inhibition rates of low-, moderate- and high-dose DGFP were 25.6%, 40.0%, 48.5%, while those of FGFP were 20.7%, 27.4%, and respectively, in vivo. As compared with those in 5-FU group, the weights together with the indexes of thymus and spleen were markedly increased （P 〈 0.01 or P 〈 0.05） after DGFP or FGFP treatment. The serum containing DGFP and FGFP obviously restrained the proliferation of H22 cells in vitro, and the inhibition rates of low-, moderate- and high-dose DGFP were 17.4%, 21.0%, and 34.5%, while those of FGFP were 16.4%, 26.3%, and 43.2%, respectively. CONCLUSION： DGFP and FGFP have conspicuous anti-tumor effects.