IL-1β、IL-6、TNF-α在兔腰椎软骨终板退变中的变化及作用

Role and change of IL-1β,IL-6 and TNF-α during rabbit lumbar cartilage endplate degeneration

[目的]研究IL-1β、IL-6、TNF-α在软骨终板退变中的作用。[方法]新西兰白兔24只随机分为实验组与对照组。实验组通过腰椎失稳而诱导了腰椎软骨终板退变动物模型。分别于术后12、24、36周摄腰椎X线片及扫描电镜观察,检测不同时段软骨终板中IL-1β、IL-6、TNF-α含量。[结果]X线片示实验组较对照组钙化明显;B rad-ner指数示椎间隙高度随时间延长而逐渐降低;扫描电镜示实验组软骨终板随时间延长胶原纤维逐渐变细、排列紊乱、出现裂隙,蛋白多糖丢失加剧,上述变化较对照组显著;实验组IL-1β、IL-6、TNF-α含量较对照组增多(P<0.05)。[结论]IL-1β、IL-6、TNF-α在软骨终板退变过程中含量相应增加,提示炎性细胞因子可能在软骨终板退变的形成和发展中起重要作用。

[ Objective] To study the role of nitric oxide on the degeneration of the cartilage endplate （CEP） . [ Method] Twenty-four New Zealand rabbits were divided into experiment and control groups. In the experiment group, the model of lumbar CEP degeneration was established by resection of all lumbar supraspinous and interspinous ligaments, excision of parts of zygapophysial joints and detachment of the posterior paravertebral muscles from lumbar vertebraes. Mechanical instability of the lumbar spine could induced the process of CEP degeneration. The X-ray examination of lumbar spine was examined by at 12, 24 and 36 weeks with or without operation, respectively. Changes of the ultrastructure of CEP were also observed by scanning electron microscope （SEM） during this process, and the content of IL-1 [3, IL-6, TNF-α in the CEP were also detected during the periods. [Result] X-ray graph showed that the CEP calcified gradually with time increasing, and those of the experiment groups calcified more obviously than those of the control groups. The SEM showed that collagen fibers became thin, irregular and fissured and the content of proteoglycan decreased severely with the elapse of time. The changes in experiment groups were more obvious than those in control groups. The content of IL-1β, IL-6, TNF-α had a significant difference between the experiment groups and the control groups （P 〈0. 05）. However, there was no significant difference between 24 and 36 weeks in the experi- ment groups （P 〉 0. 05 ）. [ Conclusion ] It implies that inflammatary cytokin may play an important role in the development and progression of the degeneration of cartilage endplate.