酶法修饰人参茎叶总皂苷及其HPLC图谱研究

Enzymatic modification of total ginsenosides in Panax ginseng stems and leaves and their HPLC chromatograms

目的研究生物酶制剂对人参茎叶总皂苷的修饰及其修饰前后HPLC图谱的变化。方法利用复酶制剂A对人参茎叶总皂苷进行酶法修饰,采用TLC法鉴别修饰前后的变化,并比较修饰前后的HPLC图谱的变化。结果在设定的积分事件下,复酶制剂A修饰人参茎叶总皂苷后色谱特征峰由34个减至16个,其中有13个特征色谱峰相对峰面积和相对峰高均明显降低,21个色谱峰检测不到相应信号,产生4个明显增加或新色谱峰。结论复酶制剂A能够有效地修饰人参茎叶总皂苷,采用HPLC图谱能够很好的监测其变化。

Objective To investigate the modification of total ginsenosides in Panax ginseng stems and leaves by biological enzyme preparations, and their HPLC chromatogram changes. Methods Complex enzyme A was used for modifing the total ginsenosides in P. ginseng stems and leaves. Changes before and after modification were determined by TLC analysis and quantificationally described with HPLC fingerprints. Results At assured integration events, numbers of chromatographic peaks were decreased from 34 to 16 after total ginsenosides in P. ginseng stems and leaves modified by complex enzyme A, among which relative peak area and height of 13 peaks were significantly reduced, the relevant signals of 21 peaks could not be detected, four new peaks appeared. Conclusion Complex enzyme A has great potential modification on total ginsenosides in P. ginseng stems and leaves and HPLC fingerprints could remarkably monitor the changes of modification on total ginsenosides in P. ginseng stems and leaves.