HPLC法测定小承气汤中大黄酸和厚朴酚的含量

Determination of the contents of rhein and magnolol in the Xiaochengqi decoction by HPLC

目的 建立小承气汤中大黄酸和厚朴酚的HPLC含量测定方法。方法 样品以体积分数为7096的乙醇回流提取。采用Thermo-C18柱（250mm×4．6mm，5μm），流动相为甲醇-体积分数为1％乙酸水溶液（体积比为72：28），流速为1．0mL·min^-1，检测波长为254nm。结果 大黄酸和厚朴酚的线性范围分别为1．52～19．0mg·L^-1和1．68—21．0mg·L^-1。平均回收率为97．696和101．396。结论 建立的方法可用于小承气汤的质量控制。

Objective To establish a reversed-phase HPLC method for determination of rhein and magnolol in Xiaochengqi decoction. （tradi tional Chinese raedi cines）Methods The sample was separated on a Thermo- C18 column （250mm×4.6mm,5μm）using methanol-1% acetic acid solution（ V： V = 72：28） as the mobile phase at a flow rate of 1.0 mL· min^- 1. The detection was set at 254 nm. Results The calibration curve was linear in the range of 1.52 - 19.0 mg·L^-1（r =0.999 7） for the rhein and 1.68 - 21.0 mg·L^-1 for the magnolol（ r = 0. 999 6）. The inner-day RSD was 0.15 % and 0.14 %, and the average was 97.6 % and 101.3 % respectively. Conclusions This method is accurate, simple and sensitive, and can be used for the quality control of Xiaochengqi decoction.