厚朴及其炮制品HPLC-DAD-MS色谱指纹图谱的研究

Chromatographic studies on fingerprints of Cortex Magnoliae officinalis and their processed products by HPLC-DAD-MS

目的:厚朴生品与炮制品色谱指纹图谱的建立及应用。方法:应用HPLC-DAD-MS法建立厚朴及其炮制品的色谱指纹图谱,色谱条件为:LC-18分析柱(5μm,250 mm×4.6 mm),柱温30℃,流速1 mL/m in,流动相为水-乙腈,梯度洗脱;测定了10批厚朴生品、10批厚朴炮制品和姜汁样品。结果:建立了厚朴生品HPLC-DAD指纹图谱,10个生品样品有22个共有峰,应用HPLC-MS对其部分主要共有峰进行了初步鉴定;同法得到了厚朴炮制品的指纹图谱,有23个共有峰,与生品比较,峰形相似,炮制品比生品增加了生姜中的有效成分姜酚。结论:在建立的分析条件下获得的色谱指纹图谱可较全面地反映厚朴的化学成分及其炮制前后的差异。

AIM ： To establish chromatogram figerprints of fresh Magnoliae officinalis and their processed product by HPLC-DAD-MS method. METHODS： A HPLC-DAD-MS method was proposed for quality assessment of Magnoliae officinalis and their processed product, the HPLC columm, mobile phase, elution mode and gradient program were optimized in order to obtain high quality HPLC profile. HPLC analysis was performed on a LC-18 colunm （5 μm,250 mm × 4.6 mm） with the mixture of H2O（A） and acetonitrile （B） as mobile phase in gradient mode. The colunm temperature was set up at 30 ℃ and the flow rate was 1 mL/min. RESULTS： The HPLC fingerprint was established based on the analysis of ten fresh Magnoliae officqinalis herbal samples. Twenty-two characteristic peaks were found in all ten samples. The main characteristic peaks were identified based on the comparison of UV and MS spectra of each analyte with that of authentic compounds and literature data. The HPLC fingerprint of processed Magnoliae officinalis was established. Comparing with corresponding fresh herbal samples, twent-three characteristic peaks were found in all ten processed herbal samples. Moreover, gingerol was found in processed Magnoliae officinalis. CONCLUSION： The fingerprint chromatograms from HPLC method can be used to evaluate the quality of Magnoliae officinalis and their processed products.