白花蛇舌草对H22肝癌细胞热休克蛋白70表达的影响

Effect of Herba Hedyotis Diffusae on Expression of Heat Shock Protein 70 in Hepatoma Cell Line H22

【目的】观察H22肝癌细胞热休克蛋白70(HSP70)表达及清热解毒中药白花蛇舌草对其表达的影响。【方法】以培养的H22肝癌细胞腹腔接种于小鼠使小鼠致瘤,然后将荷瘤小鼠分为模型组、白花蛇舌草组(剂量为25 g/kg)、党参对照组(剂量为25 g/kg)及热休克处理组共4组。采用免疫组化标记法检测各组活体腹水H22肝癌细胞HSP70的表达情况。【结果】白花蛇舌草组及热休克处理组H22肝癌细胞HSP70的表达显著性高于模型组(P<0.05或P<0.01);党参对照组H22肝癌细胞的HSP70表达与模型组比较无显著性差异(P>0.05)。【结论】白花蛇舌草可能通过诱导H22肝癌细胞HSP70表达,增强其免疫原性而发挥抗肿瘤作用。

[Objective] To observe the expression of heat shock protein 70 （HSP70） in hepatoma cell line H22 and to investigate the effect of Herba Hedyotis Diffusae （HHD） on its expression. [Methods] The tumor-bearing model mice were induced by intraperitoneal inoculation of cultured H22 ceils and then were divided into four groups： model group, HHD group, Radix Codonopsis group and heat shock group. The in-vivo expression of HSP70 in ascitic H22 cells of different groups was detected by immunohistochemical method. [ Results ] HSP70 expression in HHD group and heat shock group was higher than that in the model group （ P 〈 0.05 or P 〈 0.01 ）. The difference of HSP70 expression between Radix Codonopsis group and the model group was insignificant （ P 〉 0.05）. [ Conclusion] HHD has an antitumor effect and its mechanism is probably related to the induction of HSP70 expression and to the increase of immunogenicity in hepatoma cell line H22.