黄芪对原发肾病患儿TH1/TH2细胞平衡的影响

The effects of astragalus on T helper cells balance in childhood primary nephritic syndrome

目的:探讨黄芪治疗对肾炎患儿TH1/TH2平衡的影响。方法:运用三色荧光标记法流式细胞术检测30例黄芪治疗组肾病患儿治疗前后外周血TH1和TH2细胞百分率(%),并以同期常规治疗组患儿30例作对照。结果:黄芪组有效率高于常规治疗组X2=2·31,P<0·05。黄芪组治疗后TH1和TH2细胞百分率分别为(12·04±4·92)%和(2·81±2·65)%。常规组治疗后TH1和TH2细胞百分率分别为(9·06±4·56)%和(2·58±2·34)%,TH1/TH2比值治疗后均升高,在黄芪组患儿治疗后为(4·26±2·12),常规组治疗后为(3·51±2·27),两组治疗前后有显著差异(P<0·05),黄芪组和常规组也有显著差异(P<0·05)。结论:黄芪在体内是通过促进TH2细胞向TH2细胞迁移,逆转肾病患儿TH2细胞占优势的免疫失衡而使肾病缓解。

Objective： To investigate the effects of astragalus on TH1/TH2 balance in childhood primary nephritic syndrome（PNS）. Methods. Determination of T-helper （TH）-1 and TH2 cells percentage in 30 cases of PNS with astragalas therapy before and after by three - color flowcytometry following whole blood cells culture, compared with 30 PNS children with regale therapy before and after. Results：The percentages of Till and TH2 cells in children used astragalas therapy after were（12.04 ± 4.92） %. and （2.81 ± 2.65） %. in the patients used regule therapy after, the percentages of Till and TH2 cells were（9.06 ± 4.56） % and（2.58 ±2.34） % ,there are significantly deference in both team with therapy before and after（P 〈 0.05）, there are significantly deference in two team（P 〈 0.05）. Conclusion：The beneficial effects of astragalus can be explained by the shift from the TH2 cells to Till cells in children with primary nephritic syndrome, which is primarily caused by the relatively predominant TH2 cells in vs Till cells.