肺癌患者Ras相关区域家族1A基因启动子异常甲基化的检测

Detection of RASSF1A gene promoter hypermethylation in patients with lung cancer

目的探讨肺癌组织和外周血浆、支气管肺泡灌洗液(BALF)中Ras相关区域家族1A(RASSF1A)基因启动子异常甲基化状况及其在肺癌诊断中的价值。方法用甲基化特异PCR方法对肺癌患者癌组织、癌旁组织及相应血浆、BALF进行RASSF1A异常甲基化检测。结果45例肺癌组织中,RASSF1A基因启动子异常甲基化率为53.33%(24/45),相应血浆中RASSF1A的甲基化检出率为28.89%(13/45),BALF检出率为42.22%(19/45),而癌旁组织中的RASSF1A启动子甲基化检出率为13.04%(3/23)、正常对照血浆、非肺癌患者BALF中未检出甲基化,只检出未甲基化的RASSF1A。血浆、BALF中甲基化改变与肿瘤组织甲基化状况显著相关(P<0.01);但与患者年龄、性别、肿瘤大小、恶性程度、肿瘤分类的差异无统计学意义(P>0.05)。结论血浆、BALF中RASSF1A基因异常甲基化改变的检测在肺癌的特异诊断等方面有一定的应用价值。

Objective To investigate the status and diagnostic value of hypermethylated RASSF1A gene promoter in cancer tissue and peripheral plasma and BALF of patients with lung cancer. Methods We analyzed the hypermethylation status of RASSF1A gene in 45 lung cancer tissues,23 parscancer tissues and corresponding blood plasma and BALF by methylation specific-PCR. Results The frequency of methylation of Promoter of RASSF1A gene was 53.33% （ 24/45 ） in lung cancer,28.89% （ 13/ 45） in peripheral blood plasma,42.22% （19/45） in corresponding BALF,13.04% （3/23） in lung carcinoma adjacent tissues, respectively. No methylation had being present in normal plasma and BALF controls （P 〈 0.01 ） ,which were significantly coincident and higher in tumors than para-tumor,but it was not correlated with clinical data,such as sex,age,pathological stage, pathological type and tumor size（P 〉0.05）. Conclusion Detection of aberrant methylation change of RASSF1A promoter in plasma and/or BALF may contribute to a definite value in early prognostic in lung cancer.