转化生长因子-β1基因多态性及其血清水平与高血压病肾脏损害的关系

The relationship between the gene polymorphism and serum level of TGF-β1 in patients and renal damage caused by essential hypertension

目的探讨转化生长因子-β1（TGF-β1）基因＋869T／C多态性和血清水平与高血压病（EH）及其肾脏损害的关系。方法采用序列特异性引物聚合酶链反应（PCR-SSP），检测172例高血压病患者（其中90例伴有肾脏损害）及86例对照组TGF-β1基因＋869T／C多态性，同时采用双抗体夹心ABC-ELISA法测定其血清TGF-β1水平。结果高血压病肾脏损害组TGF-β1血清水平（50．36ng／ml±6．92ng／ml）显著高于高血压病无肾脏损害组（40．62ng／ml±5．25ng／ml）和对照组（39．52ng／ml±6．68ng／ml）；高血压病肾脏损害组＋869T／C位点TT、TC及CC型频率分布（TT型13．3％、TC型44．4％、CC型42．2％）与高血压病非肾脏损害组（TT型29．3％、TC型43．9％、CC型26．8％）及对照组（TT型32．6％、TC型44．2％、CC型23．2％）比较差异均有显著性；携带C等位基因个体EH肾脏损害的风险约是T等位基因的1．90倍（OR=1．90，95％CI：1．24-2．92）；TT、TC、CC基因型组的TGF-β1血清水平（TT型36．32ng／ml±3．05ng／ml、TC型39．87ng/／ml±3．94ng／ml、CC型51．26ng／ml±4．76ng／ml）比较有显著性差异，其中CC基因型组明显高于其它两组。结论TGF-β1基因＋869T／C多态性通过影响TGF-β1的表达而与高血压病肾脏损害存在相关关系。

Objective To analyze the association of transforming growth factor-β1 （TGF-β1） gene ＋ 869 polymorphism and serum level of TGF-β1 with essential hypertension（EH） and renal injury. Methods The polymorphism of TGF-β1 gene ＋ 869T/C was analyzed by The technology of sequence specific primers-polymerase chain reaction （PCR-SSP） was used to detect the polymorphism of TGF-β1 gene ＋869T/C in 172 EH patients （90 patients with renal damage） and86 healthy contro,and the serum leveLs of TGF-β1 were determined by enzyme-linked immunosorbent assay （ELISA）. Results serum levels of TGF-β1 in the EH with renal damage group（50.36 ±6.92 ng/ml） is significantly higher than non renal damage group and the control group （40.62 ± 5.25 ng/ml and 39.52 ± 6.68 ng/ml） ;The TGF-β1 gene ＋869T/C polymorphism in EH with renal damage（TT13.3% ,TC44.4% ,CCA2.2% ）was significantly different with the other two groups（TT29.3% ,TC43.9% ,CC26.8% and TT32.6% ,TC44.2%, CC23.2% ）. The relative risk suffered from essential hypertension with renal damage of C allele was 1.90 times to the T allele （ OR = 1.90,95% CI ： 1.24-2.92）. The senna levels of TGF-β1 in different gene type groups are different. （TT 36.32 ± 3.05 ng/ml, TC 39.87 ± 3.94 ng/ml and CC 51.26 ± 4.76 ng/ml）, highest in CC gene type group. Conclusion The gene ＋ 869T/C polymorphism and the expression of TGF-β1 are correlated with essential hypertension with renal damage.