亚硝态氮对鲤鱼种血液SOD及GSH-Px的影响

Activity of SOD and GSH-Px in the Blood of Fingerling Cyprinus carpio under Different Nitrite Concentration

研究了不同浓度的亚硝态氮对鲤（Cyprinus carpio）鱼种抗氧化能力的影响。试验鱼为体重130～250g的鲤鱼种，按水体NO2^＋-N浓度设3组共9个处理进行试验，其中对照组NO2^--N浓度低于0．01mg／L，低浓度组为0．2mg／L、0．4mg／L、0．6mg／L、0．8mg／L，高浓度组为1．5mg／L、2．5mg／L、3．5mg／L、4．5mg／L，试验期30d。结果显示：低浓度组的平均超氧化物歧化酶（SOD）活力在150～175U／mL之间；高浓度组的SOD酶活力在70～90U／mL之间，明显低于低浓度组（P〈0．05）；不同NO2^--N浓度对鲤鱼种血液中谷胱甘肽过氧化物酶（GSH-Px）活力的影响没有显著差异（P≥0．05），其平均活性在250～350U／mL之间。结论：水体中NO2^--N含量超过1．5mg／L时，鲤鱼种的抗氧化能力受到一定影响。

Effects of NO2^- - N on the anti-oxidation ability of fingerling common carp fingerling were studied in this paper. Common carps（ 130 -250 g） were divided into 3 groups： control group （ NO2^- - N concentration 〈 0. 01 mg/L）, lower concentration group which included 4 treatments （0. 2 mg/L, 0. 4 mg/L, 0. 6 mg/L, 0. 8 mg/L）, and higher concentration group which included 4 treatments （ 1.5 mg/L, 2. 5 mg/L, 3.5 mg/L, 4. 5 mg/L）. This experiment lasted for 30 days. The results showed that the average activity of SOD in lower concentration group was 150 - 175 U/mL, which was higher than the higher concentration group（70 -90 U/mL）. The average activity of GSH-Px in different group had no significant difference （P〉 0. 05 ）, which was 250 - 350 U/mL. In conclusion, the anti-oxidation ability of common carp fingerling would be affected when the concentration of NO2^- - N exceeded to 1.5 mg/L.