高效液相色谱-库仑电化学检测法定量测定小鼠血浆中CTN986

Determination of CTN986 in Mice Plasma by HPLC-ECD

目的 建立血浆中CTN986的定量测定方法，为药物代谢研究提供技术手段。方法 采用HPLC-库仑电化学色谱方法，Diamonsil^TM（钻石）C18（4．6mm×200mm，5μm）色谱柱，0．2mol·L^-1磷酸二氢钠-甲醇（60：40）为流动相，Coulochem Ⅲ电化学检测器，Model 5010 Analytical cell为检测手段，以峰面积定量。结果检测下限为150μg·L^-1，定量下限为390μg·L^-1，方法线性范围为0．390—12．5，12．5—50mg·L^-1；在1．563，6．25，12．5，25mg·L^-1 4种质量浓度平均回收率为53．43％；日内、日间平均RSD为1．03％，2．28％。结论 该方法灵敏度高，方法操作简便，可满足药动学研究的要求。

OBJECTIVE To develop a method for the determination of CTN986 in mice plasma. METHODS The separation was performed on a DiamonsilTM C18 column（4. 6 mm×200 mm,5 μm） with the mobile phase consisting of 0. 2 mol · L^-1 sodium dihydrogen phosphate-methanol（60： 40） . The compound was detected by Coulochem Ⅲ electrochemistry detector equiped with Model 5010 Analytical cell. RESULTS The limit of detection was 150 μg· L^-1 and the limit of quantification was 390 μg · L^-1. The calibration curves were linear in the range of 0. 390 - 12. 5 and 12. 5 -50 mg · L^-1. The average extraction recovery was 53.43%, the RS- Ds of inter-day and intra-day validation were below 3.0%. CONCLUSION The method is sensitive, convenient and applicable for pharmacokinetic study.