鸡内源性类J亚群禽白血病病毒gp85基因的序列分析

Sequences Analysis of Avian Endogenous ALV-J gp85-like Gene

利用J亚群禽白血病病毒gp85基因两侧的序列为引物,从正常的SPF鸡胚、肉鸡胚、良凤花鸡胚和土鸡的基因组中扩增出完整的内源性类ALV-Jgp85基因序列。这4种不同品种来源的ALV—Jgp85基因与已报道的DF1细胞的内源性类ALV-Jgp85基因的同源性依次为98．5%、96．7%、98．6%、95．2%；与ALV-J原型株HPRS-103的同源性依次为97．3%、95．2%、97．6%、94．9%；与外源性IMC株的同源性依次为90．4%、88．5%、90．7%、89．6%。这将为深入探讨内源性类gp85基因在感染ALV-J发病的过程中起何种作用奠定了基础。

Primers corresponding to the nueleotide sequences flanking the J subgroup avian leucosis virus（ ALV-J） gp85 gene were used to amplify the intact endogenous gp85-like gene from uninfected SPF embryo, broiler embryo, Liang fenghua-chick embryo and Native-chick. The endogenous ALV-Jgp85-1ike genes of four different varieties demonstrated 95.2%-98.60% identical to DF1 cell Line reported, 94. 9%-97.60% identical to strain HPRS-103 ALV-J,88.5 %-90.7% identical to strain IMC10200 ALV-J. It provides a favorable foundation for further research on endogenous ALV-Jap85-1ike gene expresses what participant acts during infecting AIV-J.