神经节苷脂GM_1拮抗剂对海马CA_1区缺血神经元形态结构变化影响的离体研究

Studie of GM_1 antagonist on the changes of hippocampal neurons morphology iu CA_1 sector after ischemia in vitro

实验观察了神经节苷脂ＧＭ１拮抗剂——霍乱毒素对应激性高血压大鼠海马脑片体外人工缺血３０、６０、１２０ｍｉｎＣＡ１区神经元形态结构的影响。缺血３０ｍｉｎ时胞体变小，胞内小空泡形成，细胞器减少，少量的线粒体和内质网肿胀。核不规则，染色质分布不均，出现边集。随缺血时间的延长胞内细胞器破碎，残存细胞器结构不清，胞核固缩，部分裂解。而霍乱毒素则加重缺血３０、６０、１２０ｍｉｎＣＡ１区神经元形态学上的改变，具有明显的神经元损害作用。

The effect of GM1 antagonist Cholera-Toxin on the changes of neuronal morphology in the hippocampal slice CA1 sector of stress-induced hypertensive rats were studied after"ischemia" 30,60,120min.In the early stage of ischemia 30min,that the body of neurons became smaller;shape irregular and cell detaching each other.Nuclei of affected neurons were pyknotic.Time-dependent morphological changes became more obvious from 60min to 120min.The number of neuron in CA1 sector decreased.Ultrastructural examination showed that majority of microvacuoles in the neuronal cytoplasm correspond to swollen mitochondria.Both smooth and granular endoplasmic reticula were dilatated.Nuclei became irregularly and chromatic aggregated along the perinuclear membrane.In the late stage of ischemia 60min to 120min the structure of neurons became obscure,a part of them reptured.There occured macrovaculation in cytoplasm and nucler shrank.While with the drug treating during ischemia,the structure of injured neuron could be exacerbated by GM1 antagonist Cholera-Toxin(0.001%).