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大鼠尾加压素II收缩大鼠肺动脉干与蛋白激酶C通路相关性研究

大鼠尾加压素II收缩大鼠肺动脉干与蛋白激酶C通路相关性研究
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摘要 目的研究大鼠尾加压素-Ⅱ（RUII）收缩大鼠离体肺动脉干环与细胞信号转导通路蛋白激酶C通道的关系。方法从雄性Sprague—Dauley大鼠中分离出肺动脉干，切成3—4mm的血管环，用RUII（10nmol／L）预收缩血管达坪台期后，加入蛋白激酶C通道阻断剂H-7，制备H-7（0．1-100μmol／L）浓度一效应舒张曲线，最后分别计算EC50和Emax。结果H-7呈浓度依赖性舒张大鼠RUII预收缩的肺动脉干-log[EC50]=5．26±0．36，Emax=（97．21±17．86）％。结论细胞信号转导通路蛋白激酶C通道的激活参与大鼠尾加压素-Ⅱ收缩大鼠肺动脉干效应。 Objective The aim of our study was to investigate the influence of protein kianse C inhibition on rat urotensin Ⅱ induced vasoconstriction. Methods The train mmeus pulmanalis was dissected from the nude Sprague - Dawley rat, artery ring width was 3 - 4mm. Inhibitor of protein kinase C channel, H- 7（0.1 - 100/μmol/L） were added into medium after rat urotensin Ⅱ（ 10nmol/L）indueed vasoconstriction had reached plateau to construct the relaxant concentration- response curves and their EC50 and Emax. Results H - 7 caused concentration - dependent relaxations of rat urotensin Ⅱ preeontracted arteries [ - log[EC50] = 5.26 ± 0.36. Emax = （97.21 ± 17.86）% of the response to 10 nmoL/L rat urotensin Ⅱ]. Conclusion Rat UII induced rat main pulmonary artery contraction is related to the activation of protein kinase C signal transduciton pathway.
作者 薛必成 陈少贤 龚永生 徐正衸 王良兴 范小芳
机构地区 温州医学院附属第一医院 温州医学院肺心病研究室 温州医学院病理生理学教研室
出处 《浙江临床医学》 2007年第1期3-4,共2页 Zhejiang Clinical Medical Journal
基金 浙江省教育厅 科学基金20020468号
关键词 尾加压素-Ⅱ 蛋白激酶C 肺动脉干 urotensin Ⅱ protein kinase C tnmcus pulmonalis
分类号 R341 [医药卫生—基础医学]
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参考文献11

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二级参考文献11

  • 1[1]Pearson D, Shively JE, Clark BR, et al. Urotensin-II: a somatostatin-like peptide in the caudal neurosecretory system of fishes[J]. Proc Natl Acad Sci USA, 1980,77(8):5021-5024.
  • 2[2]Coulouarn Y, Lihrmann I, Jegou S, et al. Cloning of the cDNA encoding the urotensin-II precursor in frog and human reveals intense expression of the urotensin-II gene in motoneurons of the spinal cord[J]. Proc Natl Acad Sci USA ,1998,95(26):15803-15808.
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  • 4[4]JD, Liu WS, Glover GI, Wilson S, et al. Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14[J]. Nature,1999,401(6750):282-286.
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  • 8[8]Maguire JJ, Kuc RE, Davenport AP, et al. Orphan-receptor ligand human urotensin-II: receptor localization in human tissues and comparison of vasoconstrictor responses with endothelin-1[J]. Br J Pharmacol,2000,131(3):441-446.
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  • 10[10]Douglas SA, Sulpizio AC, Piercy V, et al. Differential vasoconstrictor activity of human urotensin-II in vascular tissue isolated from the rat, mouse, dog, pig, marmoset and cynomolgus monkey[J]. Br J Pharmacol,2000,131(7):1262-1274.

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浙江临床医学
2007年 第1期

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