反相离子对色谱法同时测定更年灵胶囊中维生素B_1和维生素B_6的含量

Determination of Vitamin B_1 and Vitamin B_6 in Gengnianling Capsules by RP-HPLC

目的：建立更年灵胶囊中维生素B1和维生素B6的含量测定方法。方法：采用反相离子对色谱法，以甲醇-庚烷磺酸钠水溶液（15：85）为流动相，流速为1．0mL·min^-1，检测波长为270nm，柱温：35℃，维生素B1和维生素B6民理论板数大于4000。结果：维生素B6在0．258-1．935μg呈良好的线性关系，平均加样回收率为97．74％，RSD=1．11％；维生素B1在0．104～0．78μg呈良好的线性关系，平均加样回收率为98．32％，RSD=0．87％。结论：此方法快速，结果准确，操作简便，可用于含有微量维生素B1、B6的中药制剂产品质量研究。

Objective： To establish a RP- HPLC method to determine the content of Vitamin B1 and Vitamin B6 in Gengnianling capsules. Methods： The sample was determined by RP- HPLC. The mobile phase consisted of methanol -sodium sulfonic acid heptane solution （15：85） . The flow rate was 1.0mL·min^-1. The detection wave- length was at 270nm. The temperature of the column was 35℃. The number of theoretical plates of Vitamin B1 and Vitamin B6 are both above 4 000. Results： The Vitamin B6 calibration curve was linear in the range of 0. 258 - 1.935μg, the average recovery rate was 97.74%, the RSD was 1.11%. The Vitamin B1 calibration curve was linear in the range of 0. 104 -0.78μg, the average recovery rate was 98.32%, the RSD was 0.87% . Conclusion： The RP -HPLC method for determination Vitamin B, and Vitamin B6 is speediness, accurate, simple and suitable for quality studies of Chinese traditional medicine which contains little Vitamin B1 and Vitamin B6.