蒙古族原发性高血压人群与血管紧张素转换酶ACE I/D基因及血管紧张素原AGT M235T基因多态性的关系

The association between gene ploymorphisms of angiotensin-converting enzyme ACE I/D,angiotensinogen AGT M235T and Mongolian patients with primary hypertension.

目的研究血管紧张素转换酶（ACE）基因及血管紧张素原（AGT）基因多态性与蒙古族原发性高血压病的关系。方法应用PCR-RFLP技术检测96例原发性高血压患者与正常对照组108名健康受试者血管紧张素转换酶基因第16内含子I／D多态性及血管紧张素原基因第二外显子M235T多态性。结果①蒙古族人群ACE基因I／D位点II、ID、DD基因型频率在高血压组和正常血压组分别为0.44、0．38、0．18和0．42、0．32、0．26，差异无显著性（χ^2=1.693，P=0．192，OR=0．643，95％可信区间0．330-1．254）；②I、D等位基因的频率分别为0．63、0．37和0．58、0．42，差异无显著性（χ^2=0．808，P=0.363，OR=0．834，95％可信区间0．560-1．240）；③AGT M235T位点MM、MT、TT基因型的频率在高血压组和正常血压组分别为0．21、0.73、0．06和0．55、0．34、0．11，两组之间差异无显著性（χ^2=0．495，P=0．482，OR=0．681，95％可信区间0．233-1．993）。④M、T等位基因频率分别为0．58、0．42和0．72、0．28，差异无显著性（χ^2=0．051，P=0．821，OR=1．047，95％可信区间0．702-1．562）；⑤同时分析AGT基因M235T基因型与ACE基因I／D基因型时结果为它们在蒙古族人群患高血压方面无协同作用。各亚组比较高血压组与对照组均无统计学差异，P〉0．05。结论血管紧张素转换酶基因I／D基因型和血管紧张素原基因M235T基因型与蒙古族人群发生原发性高血压无关。

Objective ： To investigate the association between gene ploymorphisms of Angiotensin - Converting enzyme （ ACE）, Angiotensinogen （AGT） and Mongolian patients with primary hypertension. Methods： insertion/deletion （I/D） polymorphism in intron 16 of ACE gene and number two exon M235Tpolymorphisms of AGT gene were analyzed in 96 patients with primary hypertension. and 108 healthy adults served as control by using PCR - RFLP （restriction fragment length polymorphism）. Results： （1）II, ID and DD genotypic frequency of ACE gene I/D site were 0. 44, 0. 38 and 0. 18 in patients with primary hypertension , there were not significant difference with 0. 42, 0. 32 and 0. 26 in control groups （χ^2= 1. 693 , P = 0. 192, OR = 0. 643, 95 % CI ： 0. 330 - 1. 254）. （2）The frequency of I, D allel were O. 63, 0. 37 in patients and 0. 58, 0. 42 in control, there were not significant difference （χ^2 = 0. 808, P = 0. 363, OR = 0. 834, 95% CI： 0. 560 - 1. 240）. （3）MM, MT, TT genotypic frequency of AGT gene were 0. 21, 0. 73, 0. 06 in patients with primary hypertension and 0. 55, 0. 34, 0. 11 in control, there were not significant difference in two groups （χ^2 =0.495,P =0.482 OR=0.681, 95% CI： 0.233-1.993）. （4） The frequency of M, T allel were 0.58, 0.42 and 0.72, 0. 28, there were not significant difference in two groups （χ^2 =0. 051, P =0. 821, OR = 1. 047, 95% CI： 0. 702 - 1. 562）. （5）The result was M235T genotype of AGT gene and I/D genotype of ACE gene had no synergistic action in Mongolian people with primary hy- pertension when two gene was analyzed at the same time. Every subset comparing had no statistics difference in two groups （ P 〉 0. 05 ）. Conclusion： I/D and M235T genotype of ACE and AGT gene have no relation to Mongolian people with primary hypertension.