福建省发现1例登革3型病毒感染者

Emergence Infection of Dengue Serotype-3 Virus in Fujian,China

[目的]对福建省2006年发现的1例登革热病例进行实验室检测并鉴定病毒型别。[方法]应用免疫层析及ELISA方法检测感染者双份血清中病毒IgM和IgG抗体,逆转录-套式PCR方法扩增登革病毒特异性核酸片段,并与1～4型病毒特异性产物比较,判定其型别。[结果]双份血清标本均检出病毒IgM抗体,恢复期血清中IgG抗体出现阳转。使用通用引物和型特异性引物,在急性期标本中检出血清3型登革病毒特异性核酸,其他型别为阴性。流行病学调查表明该病人系从菲律宾回国,在当地有蚊虫叮咬史。[结论]该登革热病例为血清3型病毒感染。结合流行病学调查,其感染地可能为菲律宾,为防止病毒暴发流行,应对其进行密切监测。

[Objective] To identify one suspected case infected by dengue virus （DV） found in 2006 in Fujian. [Methods] The sera specimens in acute and convalescent phase were collected and IgM and IgG antibody detected by immunoassay. Viral nucleic acid was detected by using reverse transcript nested PCR method, and virus sero-type was identified by comparison of the PCR product from specimen and standard dengue type 1-4 virus. [Results] IgM antibody against DV presented in both sera and the IgG antibody positive-conversed in convalescent sera. The DV serotype-3 specific nucleic acid was found in the acute specimen. Epidemiological investigation revealed the patient experienced mosquito hite when traveled in Philippine. [Conclusion] The patient was infected by DV serotype-3 and may be in Philippine. It is necessary to carry out the surveillance of DV for the disease control and prevention.