可见光对培养的人RPE细胞生长的影响以及SOD和CAT的防护作用

THEEFFECTOFLIGHTONTHEGROWTHOFTHECULTUREDHUMANRPECELLSANDTHEPROTECTIONOFSODANDCAT

目的：观察外源性超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）和过氧化氢酶（ｃａｔａｌａｓｅ，ＣＡＴ）对培养的人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞光化学损伤的保护作用。方法：将铺满期原代培养的人ＲＰＥ细胞分为三组。Ａ组直接作光照处理，Ｂ组在培养液中加入ＳＯＤ（９０Ｕ／ｍｌ）和ＣＡＴ（９００Ｕ／ｍｌ）后３０分钟进行光照，Ｃ组为对照组。光照强度２４００Ｌｘ，时间６小时。处理结束后将三组细胞以２．４×１０５／ｍｌ的密度分别接种于２４孔板，每孔０．４ｍｌ。于接种后１、３、５和７天观察细胞形态，并进行细胞计数。结果：光照后３天时，Ａ、Ｂ组细胞生长缓慢，Ｃ组细胞生长活跃；３天后Ｂ组细胞生长接近Ｃ组，比Ａ组明显活跃（Ｐ＜０．０５）。结论：可见光可使培养的人ＲＰＥ细胞生长能力降低，外源性ＳＯＤ和ＣＡＴ对细胞的损伤具有保护作用。

PURPOSE:Toinvestigatetheprotectiveeffectsofexogenoussuperoxidedismutase(SOD)andcatalase(CAT)onthephoticinjuryofculturedhumanRPEcels.METHODS:Theconflu-encyprimaryculturedhumanRPEcelsweredividedintothreegroups.GroupAwasexposeddirect-lytothelightwithiluminationintensityof2400Lxfor6hours.GroupBwasalsoexposedtothelightasabove30minutesafterSOD(90U/ml)andCAT(900U/ml)wereaddedinthemedium,andgroupCwasascontrol.Afterlightexposure,thecelswereseededin24-welplatesandobservedandcountedin1,3,5and7daysafterseeding.RESULTS:ThegrowthofgroupAandBwereslowerthanthatofgruopC3daysafterlightexposure,andafterwardsthegrowthofgroupBwasmoreac-tivethanthatofgroupA(P<0.05).CONCLUSION:Thegrowthofthelight-treatedculturedhu-manRPEcelssloweddownandtheadditionofSODandCATcouldprovideprotectionagainstox-idativeinjury.