慢性阻塞性肺病患者细胞粘附分子表达研究

The study on the expression of cell adhesion molecules in chronic obstructive pulmonary diseases

对慢性阻塞性肺病（ＣＯＰＤ）患者外周血单个核细胞（ＰＢＭＣｓ）表面的ＣＤ１１ａ／ＣＤ１８，ＣＤ１１ｂ／ＣＤ１８（ＡＰＡＡＰ法）及ＣＤ４４（流式细胞分析法）粘附分子表达及血浆中可溶性Ｅ．Ｐ－选择素水平进行检测。结果：ＣＯＰＤ急性加重期患者ＰＢＭＣｓ表面ＣＤ１１ａ、ＣＤ１１ｂ及ＣＤ４４分子表达明显增高，血浆中可溶性Ｅ．Ｐ－选择素水平亦显著增高，与正常人及缓解期患者相比均有显著性差异。综合治疗可使血浆中Ｅ．Ｐ－选择素水平显著降低；ＰａＯ２与可溶性Ｅ－选择素水平呈负相关（ｒ＝－０．６７，Ｐ＜０．００５）。提示：ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ４４及Ｅ．Ｐ－选择素粘附分子表达增强参与了ＣＯＰＤ的发病过程；监测血液中可溶性Ｅ－选择素水平可反映病情变化及预后。

The expression of CD 11a ,CD 11b ,CD 44 adhesion molecules on the surface of peripheral blood mononuclear cells(PBMCs)and changes of soluble plasma E.P selectin levels were measured in patients with chronic obstructive pulmonary disease(COPD).We found that:The expression of CD 11a 、CD 11b and CD 44 molecules on PBMCs and the levels of soluble plasma E.P selectin in the severe stage of COPD patients were all highly elevated than that of normal controls and COPD patients in the stable stage.Both plasma E and P selectin levels were all decreased significantly after treatment.There existed a negative correlation between PaO 2 and soluble E selectin level( r=-0.67,P <0.005).All these indicate that:The high expression of CD 11a ,CD 11b ,CD 44 and E.P selectin molecules are involved in the process of COPD.Mornitoring soluble E selectin level in blood can reflect the disease condition and predict prognosis.