爪哇根结线虫-β1,4-内切葡聚糖酶基因cDNA全长克隆和序列分析

Cloning and sequence analysis of full-length cDNA of β-1,4-endoglucanase genes of Meloidogyne javanica

以爪哇根结线虫为材料,根据同源序列法分别进行3′末端和5′末端的RACE,获得-β1,4-内切葡聚糖酶基因cDNA全长。此cDNA全长序列为1 675 bp,包括1 521 bp的完整ORF,编码一含506 aa的多肽。该基因命名为M j-eng3-,其推导蛋白的编码氨基酸与南方根结线虫的-β1,4-内切葡聚糖酶基因M I-ENG-1、M I-ENG-3和M I-ENG-4的同源性为95.3%、95.8%和85.3%,与另外5个植物寄生线虫β-1,4-内切葡聚糖酶基因推导蛋白PP-ENG-1、GR-ENG-1、GT-ENG-1、HG-ENG-1、HS-ENG-1编码氨基酸的同源性分别为51.2%、43.7%、43.7%、46.8%和45.3%。

The full-length cDNA sequence of β-1, 4-endoglucanase gene Mj-eng-3 was obtained by RT-PCR and RACE from Meloidogyne javanica. It consisted of I 675 nucleotides, and encoded 506 amino acids. The amino acid sequence of MJ-ENG-3 was 95.3% ,95.8% and 85.3% identical with MI-ENG-1, MI-ENG-3 and MI-ENG-4 from M. incognita respectively, while it was 51.2% ,43.7% ,43.7%, 46. 8% and 45.3% identical with PP-ENG-1, GR-ENG-1, GT-ENG-1, HG-ENG-1, HS-ENG-1 from other plant nematodes, respectively.