拟南芥抗灰霉病菌相关基因的差异表达分析

Differential expression analysis of pathogensis-related genes in different Arabidopsis ecotypes to Botrytis cinerea

Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them.Three fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST program.It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B.cinerea.

Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea. Total 150 differentially expressed cDNA fragments were obtained, 49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them. Three fragments displayed high homology （96.1%, 99.6% and 100.0% ） to phosphatidylinositol transfer-like protein IV （Secl4p protein）, TAC clone K919 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana, while alignment with NCBI databases using BLAST program. It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B. cinerea.