高效液相色谱及蒸发光散射检测器法测定银杏叶片中萜类内酯的含量

Determination of Terpene Lactones in Ginkgo Tablet by HPLC and ELSD

目的测定银杏叶片中萜类内酯银杏内酯A，B，C及白果内酯的含量。方法采用高效液相色谱法（HPLC）以蒸发光散射检测器（EISD）检测，对银杏叶片中的活性成分银杏内酯A,B，C及白果内酯进行测定。结果银杏内酯A在2．12—10．6μg范围内呈良好的线性关系（r=0.9995），平均回收率为96．0％；银杏内酯B在2．10-10．5μg范围内呈良好的线性关系（r=0．9993）。平均回收率为94．6％；银杏内酯C在1．97-9．80μg范围内呈良好的线性关系（r=0．9996），平均回收率为92．4％；白果内酯在4.12～20．6峭范围内呈良好的线性关系（r=0．9998），平均回收率为93．6％。结论该方法准确可靠，可用于银杏制剂萜类内酯的质量监控。

Objective To determine the content of ginkgolide A, B, C and bilobalide in the Ginkgo tablet. Methods The HPLC method with Evaporative Light Scattering Detector （ELSD） was used to determine the content of ginkgolide A, B, C and bilobalide in the Ginkgo tablet. Results The method had good linear relationship. The average recovery were 96.0% ,94.6% ,92.4% and 93.6%, respectively. Conclusion The method is accurate and reliable, which can be used for quality control on Terpenelactones of Ginkgo tablet.