香菇多糖增强树突状细胞瘤苗的抗肿瘤作用及其机制研究

Study on Effect of Lentinan in Enhancing Anti-tumor Action of Dendritic Cytoma Vaccine and Its Mechanism

目的进一步提高树突状细胞(DC)瘤苗的抗肿瘤作用,探索有效的抗肿瘤生物疗法。方法以腺病毒为载体介导黑色素瘤抗原(gp100)转染至DC制备成DC瘤苗,研究不同剂量香菇多糖(lentinan, LNT)单独或联合gp100-DC瘤苗对荷瘤小鼠的免疫治疗作用及其相关机制。结果LNT联合gp100-DC瘤苗能有效抑制恶性黑色素瘤生长,无瘤生存率达到66．7％。LNT联合gp 100-DC瘤苗治疗后,能显著增强荷瘤小鼠的细胞毒性T淋巴细胞(CTL)和自然杀伤(NK)细胞活性,提高脾细胞的干扰素-γ(IFN-γ)和IL-2分泌水平,组织学检查发现瘤体及瘤周有大量炎性细胞浸润,肿瘤细胞发生明显坏死。结论LNT联合gp100-DC瘤苗能有效地增强DC瘤苗的抗肿瘤免疫反应。

Objective To improve the anti-tumor effect of dendritic cytoma vaccine （DCV） for finding an effective anti-tumor biotherapy, Methods DC vaccine prepared by transfection of adenovirus mediated melanoma-associated antigen gene （gp100） into bone marrow-derived dendritic cell （DC） was used to study the immuno-therapeutic effect and the mechanism of lentinan （LNT） in different dosages, used alone or combined with gp100-DC for treatment of B16 melanoma bearing mice. Results After being treated with LNT combining gpl00-DC, the growth of malignant melanoma was inhibited with the tumor-free survival in the experimental animals being 66.7 %, The treatment could also significantly enhance the activity of cytotoxicity T lymphocyte （CTL） and natural killer （NK） cells, elevate the levels of interleukin-2 （IL-2） and interferon-γ （IFN-γ） in splenocytes, and histological examination showed that a large amount of inflammatory cells infiltrated inside and around the tumor, and obvious necrosis of tumor cells was found. Conclusion By combined use with LNT the anti-tumor immuno-reaction of DCV vaccine could be enhanced effectively.