脑海绵状血管瘤遗传家系永生细胞株的建立

Establishment of immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations

目的:建立脑海绵状血管瘤家系永生细胞株.方法:利用EB病毒转化外周血B淋巴细胞,同时加入环孢霉素A的方法.结果:成功建立了该疾病家系7名成员永生细胞株,其中5名为患者.结论:脑海绵状血管瘤家系永生细胞株成功建立.

AIM： To establish immortal lymphoblastoid cell lines in a pedigree of cerebral cavernous malformations （ CCM ）. METHODS ： The cell lines were constructed by means of adding Epstein-Barr virus and Cyclosporine A onto peripheral B lymphocytes. RESULTS： We successfully established 7 immortal lymphoblastoid cell lines in the family, and 5 of them suffered from CCM. CONCLUSION： These immortal lymphoblastoid cell lines would preserve the unique genome of CCM.