慢性阻塞性肺疾病患者HO-1基因启动子微卫星多态性与iNOS表达的关系

Relationship between the microsatellite polymorphism of HO-1 gene promoter and iNOS expression in patients with chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病患者(COPD)血红素加氧酶-1(HO-1)基因启动子微卫星多态性与诱导性一氧化氮合成酶(iNOS)表达的关系。方法利用聚合酶链反应(PCR)及免疫组织化学等技术在50例肺癌合并COPD(COPD组)与30例未合并COPD(对照组)的肺组织中检测HO-1基因启动子5′端(GT)n重复序列不同基因型频率分布特征及其iNOS表达的关系。结果COPD组iNOS强阳性表达率显著高于对照组(P<0.01)。含有L型等位基因(L/L、L/M、L/S)的样本,iNOS表达强阳性率显著性高于不含有L型等位基因(M/M、M/S、S/S)的样本(P<0.01)。结论HO-1基因启动子(GT)n大量重复序列的个体可能存在着iNOS诱导性表达升高,而与COPD的易感性相关。

Objective This study investigated the relationship between the microsatellite polymorphism in Heme Oxygenase-1 （HO-1）gene promoter and expressive levels of induced nitric oxide synthase（iNOS）. Methods The relationship was analyzed by techniques of polymerase chain reaction（PCR） and immunohistochemistry in the lung tissues from 80 cases of thepatients with lung cancer, containing 50 cases of patients with COPD （ COPD group） and 30 patients without COPD （control group）. Results The showed that the stronger positive rate of iNOS expression in COPD group was significant higher than those of the control groups（ P 〈 0. 01 ） and that the stronger positive rate of iNOS expression in the subjects with a class L allele of the （GT）n repeats of the HO-1 gene promoter （L/L, L/M and L/S）was statistical higher compared with that of the subjects without a class L allele （M/M, M/ S and S/S） （P 〈 0. 01 ）. Conclusion This results suggest that there is likely an increase of the expressive levels of iNOS in the subjects of genotypes with ≥30 GT repeats in the HO-1 gene promoter region, which may he associated with the susceptibility to COPD.