大鼠睾丸限制性表达新基因Rtap2a的生物信息学分析

Bioinformatic Analysis of A Rat Novel Gene(Rtap2a)with Restricted Expression

目的为进一步研究我们克隆到的大鼠新基因Rtap2a,本研究采用多种生物信息学方法对该序列进行特征分析和功能预测,以获得该基因及其编码蛋白更多的功能提示。方法应用B1ast、ORFFinder、ScanProsite、TMpredServer和UniGene等软件或数据库,进行序列的相似性比较,开放读码框预测、染色体定位、电子表达谱和编码蛋白质的功能等分析。结果我们得到的Rtap2a序列是该基因的全长cDNA序列,在多物种间高度保守,该基因编码的蛋白可能为一N端在胞内的单次跨膜蛋白;其小鼠同源基因的电子表达谱提示Rtap2a特异地高表达于睾丸组织,在成年小鼠的睾丸中表达量最高。结论大鼠新基因Rtap2a是一睾丸限制性表达的新基因,其编码蛋白单次跨膜,进化上高度保守,可能在哺乳动物的睾丸发育和精子发生过程中具有重要功能,也有可能参与肿瘤的发生、发展等过程。因此,新基因Rtap2a有进一步研究的价值。

Objective： A rat testis eDNA expression library was screened to look for the xenogeneie homologousmolecules.Then a novel rat cDNA Rtap2a was found.d.uring the.screen.and cloned. Bioinformatic analysis was performed in order tostudy the possible function of this cDNA sequence. Methods： Various bioinformatic methods such as Blastn、ORF Finder,ScanProsite、TMpred Server and UniGene were used to analyze and predict the feature and possible functions of the novel cDNA scqoence.Results：Rtap2a was the full-length of a novel rat gene which may have conservative and basic functions in life.There was a single strong transmembrane helix withN-terminus inside predicted by TMpred Server.The homologous mouse Unigene analysis showed Rtap2a was specifically expressed in testis tissue ,and highly expressed in adult mouse testis.Conclusion：The novel gene Rtap2a is evolutionally conserved,restrictedly expresses in testis,and contains a single transmembrane region.It may function in spematogenesis and testis development and play a role during the tumor genesis and development.Theerefore,the functions of the novel gene are valuable for further investigations.