小鼠胚胎后肾移植的胚龄选择

Selection of mice embryonic metanephroi transplantation

目的:观察小鼠不同胚龄的后肾植入同种异体远交系成年宿主体内后的生长变化,分析小鼠胚胎后肾移植的胚龄选择、血管起源。方法:实验于2003-06/2004-06在锦州医学院组织与胚胎学教研室完成。实验选用普通级昆明系小白鼠24只,同笼饲养。以观察到阴道栓脱落的最早时间计为胚龄0d,以观察到仔鼠出生的最早时间计为生后0d。以随机数字表法取胚龄13,14,16d的胎鼠及生后1d的仔鼠,即胚龄13d组、胚龄14d组、胚龄16d组、生后1d组,每组8只。受体动物选择普通级体质量20g～25g的成年健康雄性小白鼠32只。对胚龄13d的小鼠胚胎离体后肾4只进行组织培养,观察10d后肾小体的发育情况。选择胚龄13,14,16d及生后1d的小鼠后肾植入同种远交系成年宿主大网膜内10d后,采用光镜及电镜技术观察后肾脏各部(特别是肾小体)的发育情况。结果:各组进行实验的后肾及宿主小白鼠32只均进入结果分析。①胚龄13d的小鼠肾脏中无成熟的肾小体,此胚龄的离体后肾经组织培养和移植后均可以生长和分化,产生成熟的肾小体。②胚龄14,16d及生后1d的小鼠肾脏中,已出现了成熟肾小体,后肾移植后则出现了排斥反应,间质水肿、出血区及淋巴细胞浸润,肾小体萎缩,甚至完全被纤维组织所取代;且排斥反应强弱与胚龄有关,胚龄越大,排斥反应越强。③培养前的后肾结构与移植前胚龄13d小鼠胚胎的后肾结构相同;培养10d后,可见出现了发育中各个阶段肾小体,电镜下可见血管球结构完整。结论:在供体后肾肾小体出现之前移植,其排斥反应轻微,生长发育良好,表明血管球形成之前是移植的最佳时间。

AIM： To observe the changes in development of embryonic metanephroi of mice at different age transplanted into adult allo-recipient of variant in the same race, and analyze the embryonic age selection and vascular genesis of embryonic metanephroi transplantation in mice. METHODS：The experiment was performed in the Department of Histology and Embryology, JinZhou Medical College from June 2003 to June 2004. A total of 24 Kunming mice were bred together. The time that pessary fell off was termed as 0 day embryonic age, and the time that young mice born was termed as 0 day neonatal age. Mice with the embryonic age of 13 （E13）, E14, E16 as well as mice with the neonatal age of 1 day were randomly divided into 4 groups E13, E14, E16 and N1 groups with 8 mice in each group. At the same time, 32 adult male mice of ordinary grade with the body mass of 20-25 g were taken as the recipients. Four embryonic metanephrois isolated from mice of E13 group were tissues-cultured, and the development of renal corpuscles were observed after 10 days. The metanephrois obtained from mice of E13, E14, E16 and N1 groups were transplanted into the adult allo- host＇omentum, and light and electron microscope techniques were used to observe the developments of different parts in metanephroi （especially the renal corpuscles） after 10 days. for mouse after transplanted into an adult allo- host＇omentum for 10 days. RESULTS： A total of 32 mice involved in all experiments entered the final analysis. ①No mature renal corpuscle was found in mice of the E13 group, and the isolated renal corpuscle could grow and differentiate after tissue culture and transplantation, in which there were mature renal corpuscles. ② Mature renal corpuscles were found in mice of the E14, E16 and N1 groups, and there were rejection, interstitial edema, infiltration in hemorrhage, homeocyte, shrank renal corpuscles after transplantation, even completely replaced by fibrous tissues. Besides, the extent of rejection was relate with the embryonic age. The order the embryonic age was, the stronger the rejection was. ③The metanephroi structure before culture was the same as that of mice in the E13 group. There were renal corpuscles in each stage of development with complete structure of glomerulus under the electronic microscope. CONCLUSION： The rejection of transplantation before mature renal corpuscles emerge in the metanephroi of recipient is slighter with better development, which indicates that it＇s the best occasion for transplantation before glomerulus emerge,