动物自体滑膜移植术治疗膝关节强直

Treatment of rabbit knee joint toughness with autogenous synovium transplantation

为探讨动物自体滑膜移植术治疗膝关节强直的疗效和安全性。于2003-10/2005-10在咸宁学院医学院外科教研室实验中心进行实验。选择60只6～10个月龄黑兔,切除膝关节外侧膝弯处关节滑膜,4周后造成人工膝关节强直动物模型,随机数字法将60只兔分为单纯松解组和滑膜移植组,每组各30只。两组均采用膝关节强直松解术,后者加自体滑膜移植术。术后第8周末,单纯松解组膝关节活动范围在0°～20°16只,20°～40°10只,40°～60°4只,60°～90°0只,滑膜移植组膝关节活动范围在0°～20°2只,20°～40°8只,40°～60°16只,60°～90°4只,两组差异有统计学意义(P<0.01)。组织学观察显示:单纯松解组第8周末和第12周末膝关节处皮下结缔组织与股四头肌之间未见滑膜组织,只见少许纤维结缔组织;滑膜移植组第8周末移植滑膜组织见少数多核巨细胞局灶性增生,间质水肿,第12周末,移植滑膜组织见血管增生,内皮细胞肿胀,间质细胞增生,有较多泡沫细胞。

To investigate the efficacy and safety of treatment of rabbit knee joint toughness with autogenous synovium transplantation, we carried out the experiment at the experimental center of Department of Surgery, the Medical .College of Xianning University between October 2003 and October 2005. Sixty black rabbits aged 6-10 months were selected and the lateral synovium of joint was resected to establish the artificial knee joint toughness models 4 weeks later. Then the rabbits were randomly divided into simple relaxation group （SR） and synovium transplantation group （ST） with 30 animals in each group. The latter group was subjected to antogenous synovium transplantation besides the relaxation of knee joint toughness. At the end of the 8^th week, the ranges of motion of knee joint of the SR group： 16 cases were 0°-20°, 10 were 20°- 40°, 4 were 40°-60°, and 0 was 60°-90°, The range of the ST group： 2 cases were 0°-20°, 8 were 20°-40°, 16 were 40°-60°, and 4 was 60°-90°, There were significantly differences between the two groups （P 〈 0.01）. The histological observation showed that at the end of the 8^th and 12^th weeks, a few fiber connective tissues but not synovium tissue between connective tissues and quadriceps were found in the SR group; in the ST group, the transplantation synovium tissue showed inflammatory proliferation multinucleated giant cell and interstitial edema after 8 weeks; After 12 weeks, vascular proliferation was found in the transplantation synovium tissue accompanying with swelling endothelial cells, interstitial cell hyperplasia and many foam cells.