桂林桃花江水体可培养中温细菌的种群及分布

Community and distribution of culturable moderate temperature bacteria from the water of Taohuajiang River,Guilin

研究了桂林桃花江水体可培养中温细菌的种群及分布.16S rDNA序列分析表明,分离获得的51株细菌主要属于-γProteobacteria(45.10%)、β-Proteobacteria(27.45%)和Firm icutes(23.53%)三大类群,其中大部分细菌与假单胞菌属(Pseudomonas)、不动杆菌属(Acinetobacter)和芽孢杆菌属(Bacillus)系统发育关系密切.共分为24个不同的分类单元,Shannon多样性指数为3.14.细菌数量在取样点26、32、33、38、42和43号较多(约105cfu/mL),在取样点9、14、20、31和35号较少(约103cfu/mL).各取样点均有假单胞菌属(Pseudomonas)分布.排污口附近分布有芽孢杆菌属(Bacillus)、不动杆菌属(Acinetobacter)和水生螺菌属(Aquaspirillum).深层洁净的水体中分布有小球菌属(M icrococcus)和色杆菌属(Chromobacterium).

Community and distribution of culturable moderate temperature bacterial from the water body of Taohuajiang River are studied. The similarity of 16S rDNA of 51 isolates is analyzed. The results indicate that the dominant bacterial groups are γ-Proteobacteria （45.10%）, β-Proteobacteria （27. 45% ） and Firmicutes （23.53%）, most of which are closely related to generus Pseudornonas, Bacillus and Acinetobacter. Diversity analysis reveals that these microbial isolates fall into 24 different taxa with the Shannon diversity index of 3. 14. The total bacterial community is abundant in sample site No. 26, 32, 33, 38 and 42 （about 10^5cfu/mL）, and less abundant in sample site No. 9, 14, 20, 31 and 35 （about 10^3cfu/mL）. With respect to the species of isolated microbes, generus Pseudomonas is distributed in each sample site; genera Bacillus, Acinetobacer and Aquaspirillum are distributed in most sewage discharges; while genera micrococcus and chromobacterium are found in deep clean water.