糖肾汤对糖基化蛋白诱导培养下系膜细胞表达金属蛋白酶组织抑制物1、2的影响

Effects of Tangshen Decoction on Expressions of TIMP1 and TIMP2 in Mesangial Cell under Induction and Cultivation of Glycosylated Protein

目的:研究糖基化蛋白诱导培养下,糖肾汤对大鼠肾小球系膜细胞表达基质金属蛋白酶组织抑制物1、2(TIMP1、TIMP2)mRNA的影响。方法:采用血清药理学及体外大鼠肾小球系膜细胞培养方法,用糖基化牛血清白蛋白(AGE-BSA)刺激肾小球系膜细胞,采用RT-PCR法检测系膜细胞TIMP1、TIMP2 mRNA表达。结果:糖基化蛋白可上调TIMP1、TIMP2 mRNA表达,糖肾汤能降低TIMP1、2mRNA表达。结论:糖肾汤对金属蛋白酶系统紊乱有一定的调节作用。

Objective： To discuss the effects of Tangshen Decoction on mRNA expressions of TIMP1 and TIMP2 in rat mesangial cell under the induction and cultivation of glycosylated protein. Methods： Serum pharmacology and vitro cultural method of rat mesangial cell were adopted, and the glycosylated protein （AGE-BSA） was used to stimulate mesangial cell. Then the mRNA expressions of TIMP1 and TIMP2 in rat mesangial cells were detected by reverse transcription - polymerase chain reaction （RT-PCR） method. Results： Glycosylated protein can up-regulation the mRNA expressions of TIMP1 and TIMP2, and Tangshen Decoction can decline the mRNA expressions of TIMP1 and TIMP2. Conclusion： Tangshen Decoction can adjust the disorder of the tissue inhibitor of metalloproteinase system.