反相高效液相色谱法测定脱氧核苷酸钠及其制剂含量

Determination of Sodium Deoxyribonucleotide and its Preparation by Reversed Phase High Performance Liquid Chromatography

目的：建立反相高效液相色谱法测定脱氧核苷酸钠及其制剂含量．方法；采用Kromasil C18色谱柱，流动相为0．02mmol·L^-1磷酸盐缓冲液（pH5．2），流速1．0ml·min^-1，柱温36℃，检测波长260nm。结果：脱氧核苷酸钠中dAMP、dGMP、dCMP、dTMP均基线分离，在250．0～15．6ug·ml^-1范围内。相关系数均为0．99914种脱氧核苷酸钠回收率在100．3％～101．1％之间。结论：该方法快速、简便、准确，制荆中辅料无干扰，可用于该药物原料及其制荆的含量测定。

Objective. To establish a HPLC method for determining the Sodium Deoxyribonucleotide and its preparation. Methods. The HPLC was performed on a column of Kromasil C18 （0. 5 um, 4. 6 × 200 mm） at 36℃ ,using phosphate buffer solution（pHS. 2）, and the detection wavelength of 260 nm. Resuts： The contents of Sodium Deoxyribonucleotide can be determined and baseline separated. A good linearity were obtained over the range of 250.0-15.6 ug · ml^-1,and the correlation coefficient of four deoxyribonucleic sodium were 0. 999. The recoveries of Sodium Deoxyribonucleotide added were 100. 3%-101. 1%. Conclusion. The method was proved to be simple, rapid and could be used for determination of deoxyribonucleic sodium and its preparation.