低剂量过氧化氢、甲醛、三氯乙烯诱导细胞适应性反应模型的建立

Establishment of adaptive response models induced by low concentration of hydrogen peroxide,formaldehyde and trichloroethylene

目的探讨低剂量的环境化学污染物过氧化氢(H2O2)、甲醛(FA)、三氯乙烯(TCE)能否诱导真核细胞的适应性反应。方法用溴化四氮唑蓝(MTT)法检测细胞存活率得到H2O2、FA、TCE对细胞毒性的剂量反应关系。按以下方式对细胞进行染毒:空白对照、低剂量、高剂量、适应性反应组(先用低剂量预刺激一定的时间后,再用高剂量进行攻击),观察细胞生长的变化,建立低剂量的H2O2、FA、TCE诱导细胞适应性反应的模型。结果0.88μmol/L的H2O2预刺激人胚肺成纤维细胞(MRC-5)24 h后,再用1 100μmol/L的H2O2刺激1 h,可以观察到适应性反应;以0.01 mmol/L甲醛预处理MRC-5细胞12 h后,再给予1 mmol/L的甲醛攻击4 h,可以观察到适应性反应;1μmol/L TCE刺激人正常肝细胞L-02 12 h后,再用30μmol/L TCE攻击24h,可以观察到适应性反应。结论低剂量的环境化学污染物H2O2、FA、TCE均可以诱导真核细胞的适应性反应。

Objective To establish the adaptive response models induced by low concentration of hydrogen peroxide （H2O2）, formaldehyde（FA） and trichloroethylene（TCE）. Methods Cells were treated with a series of doses of H2O2 ,FA and TCE. The dose-effect manners of their toxicology were acquired by means of MTT test. The dose without adverse effect or proliferation was chosen as low dose, while the dose of obvious adverse effect as high dose. Cells were treated with high dose after pretreated with low dose for a period of time,and the damage effect on cells was observed. The resistance effect of low dose pretreated to the damage effect induced by high dose was evaluated, and then the adaptive response models were constructed. Results Adaptive response models were observed and the models were as follow.（1）MRC-5 cells were attacked with 1 100 μmol/L H2O2 for 1 h after pretreated with 0. 88/anol/L H2O2 for 24 h;（2）MRC-5 cells were attacked with 1 mmol/L FA for 4 h after pretreated with 0.01 mmol/L FA for 12 h; （3）L - 02 cells were attacked with 30 μmol/L TCE for 24 h after pretreated with 1 μmol/L TCE for 12 h. CONCLUSION Low concentration of hydrogen peroxide, fon＇naldehyde and trichloroethylene could induce adaptive response of cells, which would provide ascientific basis for the mechanism of adaptive response induced by ECPs and prevention strategy.