阻塞性黄疸对肠道微生态环境影响的实验研究

Effect of obstructive jaundice on intestine microenvironment in rats

目的探讨阻塞性黄疸对肠道微生态环境的影响。方法将SD大鼠32只随机分为两组,胆管结扎组(BDL组)16只,无菌术下开腹行胆管结扎;假性胆管结扎组(SL组)16只,仅分离肝蒂而不予结扎。术后2周处死大鼠,观察血浆内毒素、回肠内粪便的pH值、回肠黏膜固有层淋巴细胞的变化,观察小肠组织结构的病理改变。结果术后2周BDL组血浆内毒素增高,大便pH值降低、肠黏膜固有层淋巴细胞IgA(+)、CD4(+)、CD8(+)明显减少,肠黏膜结构明显受损。结论阻塞性黄疸后大鼠肠内微生态环境发生明显变化,因此在治疗阻塞性黄疸的病人应该尽快解除梗阻,恢复肠道的微生态环境,同时也兼顾提高机体的免疫功能。

Objective To study the effect of obstructive jaundice on intestine microenvironment. Mothods Thirty-two SD rats were divided into two groups randomly.the bile duct ligated group（BDL-group）, in which rats were paunched and ligated bile duct complying with asepsis; Sham-ligated group（SL-group）, in which rat liver pedicels were separated but not ligated. Two weeks later, all rats were executed to observe changes of plasma endonoxin, pH of dejecta in ileum,and lymphocytes from ileum submucosul tissue. At the same time,enteromucosal pathologic changes were observed. Results In BDL group plasma endotoxin increased,pH of dejecta declined, IgA（ ＋ ）,CD4（ ＋ ）,CDS（ ＋ ） from submucosul tissues decreased evidently, and the enteromucosal structure was damaged. Conclusion Obstructive jaundice influences significantly rat intestine microenvironment. So it is necessary for patients with obstructive jaundice to relieve jaundice, resume intestine microenvironment rapidly during treatment, and improve immune function at the same time.