摘要
以郑麦9023盾片为材料,对影响小麦组织培养的2,4-D,AgNO3,蔗糖进行了研究。结果表明,培养基中附加0.5 mg/L 2,4-D有利于胚性愈伤组织的诱导,添加5 mg/L和10 mg/L的AgNO3对根苗的分化有负效应,蔗糖30 g/L对根苗的分化最有利。
In order to improve the efficiency of wheat tissue culture,the effects of 2,4 -D, AgNO3 and sucrose on callus induction and regeneration were studied by culturing the scutella from Zhengmai 9023. The results showed that the medium with 0.5 mg/L 2,4 -D was better for the inducement of embryogenic callus; additional 30g/L sucrose was beneficial to wheat green primor dium differentiation, while a supplement of 5mg/L or 10mg/L AgNO3 was negative.
出处
《河南农业科学》
CSCD
北大核心
2007年第1期23-26,45,共5页
Journal of Henan Agricultural Sciences
基金
河南省杰出人才创新基金项目(0121000700)
河南省自然科学基金项目(0611032100)