影响小麦组织培养效率的重要因素研究

Important Factors Affecting Wheat Tissue Culture Efficiency

以郑麦9023盾片为材料,对影响小麦组织培养的2,4-D,AgNO3,蔗糖进行了研究。结果表明,培养基中附加0.5 mg/L 2,4-D有利于胚性愈伤组织的诱导,添加5 mg/L和10 mg/L的AgNO3对根苗的分化有负效应,蔗糖30 g/L对根苗的分化最有利。

In order to improve the efficiency of wheat tissue culture,the effects of 2,4 -D, AgNO3 and sucrose on callus induction and regeneration were studied by culturing the scutella from Zhengmai 9023. The results showed that the medium with 0.5 mg/L 2,4 -D was better for the inducement of embryogenic callus; additional 30g/L sucrose was beneficial to wheat green primor dium differentiation, while a supplement of 5mg/L or 10mg/L AgNO3 was negative.