外源激素在玉米离体培养中的作用研究

Function of Exogenous Hormones on Maize Tissue Culture in Vitro

以玉米自交系黄早4、综31的幼胚为材料,对外源激素在玉米离体培养中的作用进行了研究。结果表明,2.0 mg/L的2,4-D可以诱导出高频率的胚性愈伤组织,而添加高浓度的6-BA不利于胚性愈伤的诱导;6-BA(0.5 mg/L)+KT(1.0 mg/L)+NAA(0.2 mg/L)的激素组合对于胚性愈伤组织的分化明显优于其他的激素组合;单独使用1.0 mg/L的NAA有利于根的生长和发育,虽然NAA1.0 mg/L+多效唑2.0 mg/L的激素组合中,根的平均数量和平均长度不如单独使用1.0 mg/L的NAA,但是幼苗的移栽成活率二者无显著差异。

The young embryos of maize inbred lines Huangzao4,Zong31 were cultured in vitro and the important functions of plant hormones were studied. The results indicated that medium for high frequency embryogenic callus was MS＋2.0 mg/L 2,4 -D; MS＋ 6 -BA 0.5 mg/L＋KT 1.0 mg/L＋NAA 0.2 mg/L was better for the callus differentiation; MS＋1. 0 mg/L NAA was good for root development and got almost the same survival rate as MS＋NAA1.0mg/L＋MET 2.0mg/ L did.