白桦MADS基因的克隆及序列分析

Cloning and Sequence Analysis of MADS Gene of Betula platyphylla

在分析了欧洲白桦MADS3与拟南芥AP1中的保守性后,设计了一对引物。以白桦雄花芽为材料,从总RNA中通过RT-PCR分离得到一个MADS基因,其cDNA长738bp,包含了基因完整的编码序列,白桦MADS基因与已发表的欧洲白桦MADS3基因仅相差6个碱基,同源性高达99％;推导氨基酸序列的差异为5个,同源性达97％。分析其氨基酸序列表明,存在典型的MADS MEF2转录因子结构域和K-box结构域。

A pair of primers was designed based on the analysis of MADS3 from Betula petulla and AP1 from Arabidopsis thaliana. One MADS gene was cloned from total RNA using the male bloom of B. petulla as material. DNA sequencing analysis showed that the size of the cloned MADS eDNAs were 738 base pairs, which carried the entire coding sequences. Comparison analysis exhibited differences in only six base pairs between the cloned MADS and the reported, and sequence homology reached ninetynine percent, which resulted in an alteration of five deduced amino acids and ninety-seven percent of sequence homology. Moreover, the DNA sequencing analysis showed a typical transcriptional factor of M.ADS MEF2 and K-box domain.