多穗柯中二氢查耳酮的提取、纯化与含量测定

Isolation and determination of dihydrocharcone in lithocarpus polysachyus pehd

以多穗柯(LithocarpuspolystachyusRehd)叶为原料,研究了二氢查耳酮提取及纯化的适宜条件。结果表明,二氢查耳酮提取的适宜条件为:以1∶8的料水比,在80℃条件下提取60min;二氢查耳酮纯化的适宜条件为:以聚合氯化铝为絮凝剂,在pH值为7·5,温度为55℃时絮凝40min;以ADS-7树脂为吸附剂,以100mg/mL为上样液浓度,80%乙醇为洗脱液浓度。二氢查耳酮的提取率达5·26%。用高效液相色谱法检测,二氢查耳酮含量为98·37%。

Dihydrocharcone as the target, studied the proper process for extracting and purifying Dihydrocharcone from Lithocarpus polystachyus Rehd. The result indicates the best extracting conditions are： when the solvent is water the weight of the water is 8 times that of the Lithocarpus polystachyus Rehd, the temperature of the water is 60℃, the period of the extraction is 60 minutes; the best flocculate is Polyaluminum Chloride, the best sorbent is ADS - 7 macroporous resin, then the content of dihydrocharcone can reach 5. 26%. Use HPLC to check, the pure of the dihydrocharcone is 98. 37%.