脂质体干扰素α对小鼠Lewis肺癌转移及脾细胞增殖的影响

Effects of interferon-αliposome on lung metastasis of Lewis lung carcinoma and proliferation of splenocytes in mice

Ｃ５７ＢＬ／６Ｎ小鼠尾静脉注射Ｌｅｗｉｓ肺癌细胞后，腹腔注射脂质体干扰素α（Ｌ－ＩＦＮ－α）和未包裹干扰素α（ＩＦＮ－α），连续１０ｄ；测小鼠肺湿重、肺转移结节数和ＣｏｎＡ诱导的脾细胞ＤＮＡ掺入量。结果ＩＦＮ－α５、２０万ｕ·ｋｇ（－１）组，小鼠肺湿重较对照组减小１４．１、１９．６％，肿瘤肺转移结数减少３２．７、５０．０％，ＣｏｎＡ刺激的脾细胞ＤＮＡ掺入量增加２４．６、４６．８％。１／１０剂量的Ｌ－ＩＦＮ－α可产生相同的药理活性。小鼠环磷酸胺５０ｍｇ·ｋｇ（－１）ｉｐ３ｄ后，尾静脉注射瘤细胞，实验处理同上。结果Ｌ－ＩＦＮ－α和ＩＦＮ－α对环磷酰胺处理小鼠的Ｌｅｗｉｓ肺癌细胞肺转移的抑制作用更为明显。本文结果表明，ＩＦＮ－α可抑制小鼠Ｌｅｗｉｓ肺癌转移，促进ＣｏｎＡ刺激的脾细胞增殖，并与剂量明显相关。脂质体包裹ＩＦＮ－α可使其生物活性提高约１０倍。对免疫功能受抑小鼠作用更为显著。

C57BL/6N mice were administrated iv with Lewis lung carcinoma cells 1×105,and then ip interferon-α liposome (L-IFN-α)or interferon(IFN-α)for ten days.The lung metastasis foci and proliferation of splenocytes in mice were observed. The results showed that IFN-α inhibited the lung metastasis of Lewis lung carcinoma and increased proliferation of splenocytes in miceIp 1-20×104u·kg-1 of IFN-α for 10d, lung weight of mice decreased by 3.1-18. 8%;the numbers of metastasis foci decreased by 8.2-49.9%;and [3H] TdR incorporation increased by 1.8-46.7%.After envelopment of IFN-α by liposome, its potency of anti-lung-metastasis of Lewis lung carcinoma and promoting proliferation of splenocytes increased 11 times as against unenveloped IFN-α. L-IFN-α had a more powerful effects of anti-metastasis and increasing proliferation of splenocytes in the mice administrated previously with cyclophosphamide than in the unadministrated mice.These results indicate that IFN-α liposome is a prospective preparation.