西安城区食管癌患者P53蛋白表达及其相关因素

Overexpression of P53 and its risk factors in esophageal cancer in urban areas of Xian

目的 研究西安城区常住居民食管癌的危险因素，并探讨食管癌组织中Ｐ５３的表达情况，以及诱发ｐ５３基因改变的相关因素。方法于１９９４年８月－１９９５年１月进行了病例对照研究，共调查了８９例食管癌患者和９７名正常人。用调查表调查了吸烟、饮酒、食管癌家族史等相关因素，并对其中６５例食管癌的肿瘤组织及癌旁正常组织，以及２４例对照的正常食管组织进行了免疫组化研究。统计方法采用单因素分析和多因素非条件Ｌｏｇｉｓｔｉｃ回归模型拟合分析。结果病例对照研究发现，吸烟和食管癌家族史是西安城区食管癌的主要危险因素，ＯＲ值分别为３．２６和１０．４８。免疫组化分析发现，有５２．３％（３４／６５）的肿瘤组织和６．１％（４／６５）的癌旁正常组织存在Ｐ５３蛋白的高表达，而且Ｐ５３高表达与吸烟和食管癌家族史密切相关ＯＲ值分别为３．８９和１７．２８。结论吸烟和食管癌家族史是西安城市居民食管癌的危险因素。食管癌的发生可能是在环境因素和遗传因素的共同作用下，引起了ｐ５３基因的改变所致。

Objective To investigate the risk factors of esophageal cancer (EC) in urban areas of Xi' an , and to correlate these risk factors with overexpression of P53 in EC. Methods A hospital-based case-con- trol study was performed. All cases ( 89 ) and controls(97 ) were permanent residents in urban areas of Xi'an. Tumor tissues and normal tissues adjacent to tumors of 65 cases , and 24 available normal esophageal tissues of controls were detected by P53 overexpression via immunohistochemical method. Results Smoking and family history of EC were significantly assotiated with EC in Xi' an inhabitants. OR was 3. 26 and 10. 48 , respectively. The laboratory findings indicated that P53 positive stain in Ec was 52. 3% (34/65) and 6. 1% (4/65) in normal tissues adjacent to tumor, but no positive stain was found in normal esophageal tissues of controls. Moreover , the results showed that P53 overexpression was closely related to smoking and family history of EC. OR was 3. 89 and 17. 28 , respectively, Con- clusion These findings suggest that smoking and family history of EC are important risk factors for EC , and the alteration of P53 gene may be related to smoking and inherited factor.