IL－2－PE40对小鼠异基因骨髓移植的影响

Effect of IL-2-PE40 on allogeneic bone marrow transplantation in mice

探讨白细胞介素２（ＩＬ－２）与绿脓杆菌外毒素组成的融合蛋白──ＩＬ－２－ＰＥ４０对小鼠异基因骨髓移植的影响。实验结果表明体外应用ＩＬ－２－ＰＥ４０能够选择性抑制混合淋巴细胞培养（ＭＬＣ）中的异基因反应细胞，清除ＭＬＣ诱导产生的细胞毒效应细胞，保留未活化细胞对ＣｏｎＡ诱导的丝裂原反应；体内应用ＩＬ－２－ＰＥ４０能延长异基因骨髓移植小鼠的存活期，而对ＣＦＵ－ＧＭ产率无明显影响。

IL-2-PE40 is a recombinant chimeric toxin designed to kill cells with IL-2 receptors.The in vitro experimental results showed that IL-2-PE40 was able to selectively inhibit alloresponsive cells in allogeneic mixed lymphocyte culture(MLC),eliminate cytotoxic effector cells generated in MLC,and maintain the ConA inducing mitogenic response of the nonactivated cells.The growth of CFU-GM did not inhibited by IL-2-PE40.Administration of IL-2-PE40 to mice underwent allogeneic bone marrow transplantation could prolong their survival.