摘要
简要介绍了荧光共振能量转移(FRET)原理和荧光定量PCR中常用的荧光检测方法,着重讨论了FRET应用于PCR荧光检测应注意的几个问题,并指出了未来研究趋势。
The theory of fluorescence resonance energy transfer(FRET) and methods of fluorescence detection in fluorescent- quantitative polymerase chain reaction(FQ-PCR) are introduced in this article. Applications of FRET in fluorescence detection of PCR are emphatically discussed, and FRET research progress and future trends are pointed out too.
出处
《中国医疗器械杂志》
CAS
2007年第1期39-43,共5页
Chinese Journal of Medical Instrumentation
基金
上海市重点学科建设项目(P0502)上海市教委青年基金项目(03YQHB)资助