墨西哥湾扇贝初级卵母细胞发育及卵黄发生超微结构的初步研究

The primary oocyte development and ultrastructure of vitellogenesis in bay scallop,Argopecten irradians concentricus

采用电子显微镜术,研究了墨西哥湾扇贝Argopecten irradians concentricus的初级卵母细胞发育和卵黄发生。结果表明,墨西哥湾扇贝初级卵母细胞的发育可分为卵黄合成前期、卵黄合成期和成熟期;5种细胞器,即线粒体、高尔基液泡、糙面内质网、多泡小体和微吞饮泡参与了初级卵母细胞卵黄颗粒的形成,其中,线粒体和高尔基液泡是形成卵黄颗粒的主要胞器。卵黄物质的来源有内源性和外源性两种方式,外源性卵黄物质主要来自消化腺中贮存的营养。另外,研究发现核膜泡可能参与了多泡小体的形成。

The oocyte development and vitellogenesis were observed in bay scallop, Argopecten irradians concentricus under electronic microscope. Results showed that the oocyte development of the scallop was divided into three stages： previtellogenic, vitellogenic and mature oocytes; five kinds of organelles, i.e. mitochondria, Golgi vesicles, rough endoplasmic reticulum, muhivesicular bodies and mucropinocytotic vesicles were involved in formation of yolk granules, in which mitochondria and Golgi vesicles were the key organelles. Granule materials in oocytes were both endogenous and exogenous and the exogenic nutrients were mainly derived from digestive gland. It appears that the nuclear membrane takes part in formation of multivesicular bodies.