用复合酶酶解—热水浸提法提取裙带菜孢子叶粉岩藻聚糖硫酸酯的工艺研究

The extracting technology of fucoidan from sporophyll powder of sea mustard(Undaria pinnatifida) suringar by enzymatic hydrolysis

采用正交试验方法,研究了用戊聚糖复合酶及复合纤维素酶酶解—热水浸提法提取裙带菜Undariapinnatifida孢子叶粉中岩藻聚糖硫酸酯的酶解工艺参数。结果表明:用戊聚糖复合酶酶解裙带菜孢子叶粉的最佳酶解参数为酶加量0.12%,温度为50℃,pH为5.5,时间为30 min,岩藻聚糖硫酸酯粗提物得率为5.171%,总硫酸根的质量分数为1.432%,粗提物中糖的质量分数为21.38%;用复合纤维素酶酶解裙带菜孢子叶粉的最佳酶解参数为酶加量0.227%,温度为45℃,pH为4.5,时间为70 min,岩藻聚糖硫酸酯粗提物得率为5.720%,总硫酸根的质量分数为1.118%,粗提物中糖的质量分数为28.24%。用两种复合酶酶解—热水浸提法提取的岩藻聚糖硫酸酯粗提物得率均较水煮法(4.665%)高。

The parameters of the two enzymatic hydrolyzing technology with hot water were determined in extracting fucoidan from sporophyll powder of sea mustard Undaria pinnatifida. The optimum factors were determined for Novozymes Viscozyme L through the orthogonal test： the Novozymes Viscozyme L added 0. 12%, temperature 50℃, pH 5.5 and 30 minutes. The yield rate of fucoidan extracted was 5. 171% under the optimal conditions, in which the content of SO4^2- was 1. 432% , and the content of polysaccharides was 21.38%. The optimum factors for Celluclast BG were as the following： the Celluclast BG added 0. 227%, temperature 45℃, pH 4.5 and 70 minutes,the yield rate of fucoidan extracted being 5. 720%, in which the content of SO4^2- was 1.118%, and the content of polysaccharides was 28. 24%. The yield rate of fucoidan extracted was higher by enzymatic hydrolysis with hot water extracting technology than by the traditional extracting technology（4. 665% ）.