银杏苦内酯B对重症急性胰腺炎大鼠血浆TNFα的影响

Effect of ginkgolide B on plasma level of tumor necrosis factor alpha in severe acute pancreatitis rat model

目的观察重症急性胰腺炎(SAP)大鼠血浆中肿瘤坏死因子α(TNFα)的水平变化,探讨银杏苦内酯B(BN52021)对SAP的治疗作用。方法选用Wistar大鼠45只,随机分成SAP模型组(SAP,n=15),BN52021治疗组(BN,n=15)和阴性对照组(NC,n=15)。前两组以5%牛磺胆酸钠逆行注入主胰管制成SAP模型,制模15 min后,SAP组经股静脉以0.5 ml/100 mg注射生理盐水;BN组以BN52021(5 mg/kg)代替生理盐水静注;NC组仅做剖腹术。制模后1 h,6 h和12 h分别采血,应用ELISA技术测定血浆TNFα水平。结果在1 h时相点,SAP组血浆TNFα水平(420.67±262.22 pg/ml)与BN组(403.88±177.92 pg/ml)、NC组(369.62±39.73 pg/ml)相比,均不具有显著性差异(P>0.05);在6 h1、2 h时相点,SAP组血浆TNFα水平(856.33±207.25 pg/ml,961.56±415.49 pg/ml)较NC组(369.14±16.28 pg/ml,416.43±26.54 pg/ml)明显升高(P<0.05);BN组(415.15±88.08 pg/ml,692.06±69.31 pg/ml)较SAP组明显降低(P<0.05)。结论实验证明SAP大鼠血浆TNFα水平明显升高,BN52021对SAP大鼠具有明显的治疗效果,使其血浆TNFα水平显著下降。

Objective To observe the plasma level of tumor necrosis factor alpha （TNFα）in severe acute pancrestitis（SAP） rat model and the efficacy of ginkgolide B（BN5 ,2021）in treating SAP.Methods Wistar rats were randomly assigned to model group（SAP, n = 15）, treatment gronp（BN, n = 15）, and negative control gronp（NC, n = 15）.SAP was induced by retrograde infusion of 5% sodium taurochohte into the pancreatic duct of Wistar rats. SAP group received the femoral vein injection of isotonic sodium chloride 0.5 ml/100mg 15 minutes after induction of SAP; BN group received BN52021 （5 mg/kg） ,while NC group received only abdominal incision.At 1,6 and 12 hours after operation, rats were sacrificed for determination of plasma TNFα by ELISA.Results At 1h post-operation, there was no significant difference in the plasma TNFα levels between SAP group（420.67 ± 262.22 pg/ml）,BN group（403.88 ± 177.92 pg/m]）, and NC group（369.62 ± 39.73 pg/m]） （ P 〉 0.05）. At 6 h and 12 h post- operation, the plasma levels of TNFα in SAP group（856.33 ± 207.25 pg/ml and 961.56 ± 415.49 pg/ml, respectivdy） were significantly higher than than in NC group （369.14 ± 16.28 pg/ml and 416.43 ± 26.54 pg/ml,respectively）（ P 〈 0.05） and BN group（415.15 ± 88.08 pg/ml and 692.（16 ± 69.31 pg/ml, respectvely） （ P 〈 0. 05）. Conclusions The plasma level of TNFα in SAP group is significantly increased in SAP and that in SAP rats treated with ginkgolide B is significantly decreased.