高碘对小鼠额皮质及海马组织NOS阳性神经元的影响

Effect of excessive iodine on NOS positive neurons in frontal cortex and hippocampus of mice

目的观察高碘对小鼠额皮质及海马NOS阳性神经元的影响。方法昆明种小鼠(雌雄各半12只)分为适碘与高碘组,分别喂以碘浓度为50μg/L,5000μg/L的蒸馏水6个月,作Morris水迷宫行为学测试后以还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组化法显示小鼠额皮质及海马组织NOS阳性神经元。结果与适碘组相比,高碘组小鼠脑额皮质、海马NOS阳性神经元密度显著降低,且染色变浅;行为学测试中小鼠逃避潜伏期显著延长。结论额皮质及海马NOS阳性神经元减少可能是高碘时中枢神经系统功能损伤的病理基础。

[Objective] To study the effect of excessive iodine on NOS positive neurons in frontal cortex and hippocampus of mice. [Methods] Mice, divided into two groups at random （control group with appropriate iodine and trial group with excessive iodine）, were fed with distilled water containing iodine 50 p.g/L and 5 000 Ixg/L respectively. Rats were killed at 6 months after Morris water maze. NADPH-diaphorase histochemistry was used to visualize NOS positive cells in frontal cortex and hippocampus. [Results] The mean escape latency increased and NOS positive cells decreased significantly in excessive iodine group than those in control group. [Conclusion] The reduce of NOS positive cells in frontal cortex and hippocampus may be related to the injury of cognition in excessive iodine rats