空间排斥色谱法在酶学中应用的研究进展

Research Advances of Steric Exclusion Chromatography in Application of Enzymology

空间排斥色谱法是一种高分子聚合物的柱分级分离技术,通过这种方法可以使柱中溶解的聚合物分子按其大小被分离。空间排斥色谱法在生物化学、高分子化学、无机化学、医学、临床等领域内得到了广泛的应用。本文对空间排斥色谱法的发展历程、基本原理、常用凝胶的性质等进行了详尽的论述。着重介绍了空间排斥色谱法在酶的脱盐、酶溶液的浓缩、酶的分离与纯化、酶的分子量测定中的应用,它已成为测定高聚物分子量分布的重要工具。最后指出空间排斥色谱法结合其它检测器将代替传统空间排斥色谱法,加速空间排斥色谱法不断向快速、高效方向发展。

Steric exclusion chromatography （SEC） is a technology of column graded separation for high polymer, with which the soluted polymer in column can be separated according to the difference of molecular weight. Steric exclusion chromatography, such as biochemistry, macromolecule chemistry, abiochemistry, medicine and clinic, and so on, was widely used. In this paper, the development and priniciple of steric exclusion chromatography and the Gel characters were expounded carfully, especially the application of steric exclusion chromatography in the dissalt, separation,inspissation and purification Of enzyme and molecular weight determination were reviewed.Finally,it points out that steric exclusion chromatography integrated with detector will replace traditional steric exclusion chromatography gradually to speed up auto development towards speediness and high efficiency.