摘要
采用Ａｌｅｎ打击法造成Ｃ６，７损伤，采用鞘内注射血小板活化因子（ＰＡＦ）及静脉注射ＰＡＦ受体拮抗剂ＢＮ５２０２１，观察其对颈髓组织ＡＴＰ酶活性、离子含量、含水量的影响。结果显示，ＰＡＦ能够增加颈髓损伤后颈髓组织含水量、Ｎａ＋、Ｃａ２＋含量，抑制Ｃａ２＋－Ｍｇ２＋ＡＴＰ酶、Ｎａ＋－Ｋ＋ＡＴＰ酶活性，降低伤后颈髓组织Ｋ＋、Ｍｇ２＋含量；ＢＮ５２０２１能够降低伤后颈髓组织含水量，维持Ｃａ２＋－Ｍｇ２＋ＡＴＰ酶、Ｎａ＋－Ｋ＋ＡＴＰ酶活性、Ｎａ＋、Ｋ＋、Ｃａ２＋、Ｍｇ２＋的相对稳定，提示ＰＡＦ及其受体在颈髓损伤后颈髓水肿的病理生理过程中起重要作用，ＰＡＦ受体拮抗剂能够有效地减轻外伤性颈髓水肿。
C6,7spinalcordinjurymodelswereproducedincatsbyAlen’smethod.Plateletactivatingfac tor(PAF)andPAFreceptorantagonistBN52021wereadministeredbyintrathecalorintravenousinjectionre spectively.TheirefectsonATPaseactivity,ioncontentsandwatercontentofcervicalspinalcordwereob served.TheresultsshowedthatPAFcouldincreasewatercontent,Na+,Ca2+levelsanddecreaseK+,Mg2+levels,inhibitNa+-K+ATPase,Ca2+-Mg2+ATPaseactivityofcervicalspinalcordaftertrauma;BN52021coulddecreasewatercontentandsustaintherelativestabilityofNa+-K+ATPase,Ca2+-Mg2+ATPaseac tivity,Na+,K+,Ca2+,Mg2+levels.TheresultssuggestthatPAFanditsreceptorplayanimportantroleinthepathophysiologicalprocessoftraumaticcervicalspinalcordedema,whilePAFantagonistcanefectivelyim provetraumaticspinalcordedema.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第12期787-787,共1页
Chinese Journal of Orthopaedics
关键词
脊髓损伤
ATP酶
脊髓水肿
血小板活化因子
CervicalspinalcordinjuryATPaseenzymeCervicalspinalcordedemaPlateletactivat ingfactor
