无琼脂培养诱导非洲菊无菌生根研究

Study on the Rooting of Gerbera jamesonii in Vitro in media without Agar

培养成本高,移栽成活率较低一直是植物组织培养中的两大难题。为降低组织培养中琼脂培养的成本,提高移栽成活率,需寻求一种廉价而操作简便的基质取代琼脂。以非洲菊的无菌芽为试材,分别接种在以砂砾、海绵、琼脂为支撑材料的培养基中诱导生根,并统计移栽成活率。结果显示,以砂砾为支撑基质诱导非洲菊生根快,且生根率高(分别为96.55%、89.47%、94.11%),幼苗长势最好,移栽成活率最高(分别为97.63%、96.25%、78.18%)。同时,营养液体积及支撑材料处理等细节技术对生根也有影响。说明砂砾与海绵等无机基质可以取代琼脂作组织培养的支撑物,该技术将具有较强的推广价值。

There are two tough problems in tissue culture of plants. One is high cost, another is low survival rate of the transplant. In order to reduce the cost in tissue culture with agar, and to increase the survival rate of the transplant, another kind of support in ctdture media instead of agar is needed, which is cheap and can be washed down easily, we respectively put the buds of Gerbera jamesonii in media（MS＋BA15mg.L^-1＋IBA0.2mg.L^-1 ）with three kinds of support in vitro to induce to root, which are grit, sponge, and agar. And then we transplanted the tittle rooted buds from the bottle to the bed. At the same time, the rate of root and the survival rate of the transplant were counted. The results are showed： The root rate was highest in media with grit（which were respectively 96.55%. 89.47 %. 94.11% with grit, sponge, and agar）, and the growth situation was best; Furthermore, its survival rate of the transplant was highest（which were respectively 97.63%, 96.25%. 78.18%in grit, sponge, and agar）. Moreover, there were some details affecting the results such as the liquid volume, and the way to deal with the support materials. The success in the root culture of Gerbera jamesonii without agar shows that grit and sponge can be used instead of agar. If the technique can be widely used in tissue culture of other plants, it will be great meanful in the field of plants.